Bio 210L Microbiology Lab Manual

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Useful Information
Lab safety procedures 
Checklists
Map of the lab 
Manual organization
The microscope
Volumes on the pipets
Streak plate diagram

Exercises: 
1. Microscopy and staining
2. Fungi: mold and yeast 
3. Protozoans 
4. Viruses 
5. Aseptic technique and preservation of cultures 
6. Wine and microbial quantification 
7. Sampling the environment 
8. Ames test 
9. Anaerobic growth 
10. Yogurt 
11. Bacterial characteristics: 
     • A: Differential and selective media 
     • B: Biochemical tests 
     • C: Environmental ranges 
12. Disinfectant evaluation
13. Antibiotic sensitivity
14. Physical control of microbes using heat
15. Bacterial growth curve 
16. Enrichment of purple non-sulfur bacteria (PNSB)
17. Enrichment of streptomyces 
18. Human microbial flora
19. Epidemic simulation 
20. MMWR assignment 
21. Probiotics 
22. Biofilms 
23. Dental caries 
24. Food born gastroenteritis and kit identification 
25. Water quality tests

Useful Information
Lab safety procedures 
Checklists
Map of the lab 
Manual organization
The microscope
Volumes on the pipets
Streak plate diagram

Exercises: 
1. Microscopy and staining
2. Fungi: mold and yeast 
3. Protozoans 
4. Viruses 
5. Aseptic technique and preservation of cultures 
6. Wine and microbial quantification 
7. Sampling the environment 
8. Ames test 
9. Anaerobic growth 
10. Yogurt 
11. Bacterial characteristics: 
     • A: Differential and selective media 
     • B: Biochemical tests 
     • C: Environmental ranges 
12. Disinfectant evaluation
13. Antibiotic sensitivity
14. Physical control of microbes using heat
15. Bacterial growth curve 
16. Enrichment of purple non-sulfur bacteria (PNSB)
17. Enrichment of streptomyces 
18. Human microbial flora
19. Epidemic simulation 
20. MMWR assignment 
21. Probiotics 
22. Biofilms 
23. Dental caries 
24. Food born gastroenteritis and kit identification 
25. Water quality tests