Biochemistry Laboratory Manual

Introduction to Biochemistry Laboratory
Lab Safety
Guidelines for Laboratory Notebooks

Experiments 
Experiment 1 Preparation of Buffers
Experiment 2 Titration of an Amino Acid
Experiment 3 Spectrophotometric Determination of Tryptophan and Tyrosine in Chymotrypsinogen-A
Experiment 4 Protein Determination Using Coomassie Brilliant Blue G-250
Experiment 5 Gel Filtration on Sephadex
Experiment 6 Molecular Modeling of Invertase (B-Fructofuranosidase)
Experiment 7 Preparation of Standard Plot for Invertase Activity Assay
Experiment 8 Purification of Invertase
Experiment 9 Column Profile of Invertase Elution
Experiment 10 Steady-State Kinetics Assay of Invertase
Experiment 11 Inhibition of Invertase

Appendices
Appendix 1 Care and Use of Analytical Balance
Appendix 2 Calibration and Use of Thermo Fisher Scientific Orion Star A111 pH Meter
Appendix 3 Use of Thermo Fisher GeneSys 180 UV-Visible Spectrophotometer
Appendix 4 Proper Care and Use of Mohr Pipettes and Micropipettes
Appendix 5 Perl Script to Identify Frustrated Residues in Protein