Biological Inquiry: General Biology I Laboratory Manual
Edition: 1
Copyright: 2018
Pages: 140
Edition: 1
Copyright: 2018
Pages: 140
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This laboratory emphasizes hypothesis-driven experiments and connections to real-world problems and applications. These experiments will increase your interest in learning about biology and reinforce and complement the important concepts you will explore during General Biology I lecture.
In this laboratory manual, brackets have been used to identify content from other Kendall Hunt authors. The number to the right of the bracket corresponds to the sources listed within the References and Credits section.
Preface
Contributing Faculty
Introduction to Biology Laboratory Safety Rules
Introductory Module: The Nature of Science and Experimental Design
Activity 1: Experimental Design
Graphs
How to Make a Graph
Types of Graphs
Activity 2: Interpreting Data and Graphing
Activity 3: Identifying Parts of a Scientific Experiment
Activity 4: Calculating Arm Span to Height Ratio
Activity 5: Investigating the Scientific Literature
Module 1: Macromolecules
Macromolecules
Building Up and Breaking Down Macromolecules
Part 1: Qualitative Detection of Macromolecules
Qualitative Detection of Carbohydrates
Experiment 1: Monosaccharides Using Benedict’s Test
Experiment 2: Qualitative Detection of Polysaccharides (Starch) Using Iodine’s Test
Experiment 3: Qualitative Detection of Monosaccharides and Polysaccharides (Starch) in a Food Item (Example: Ready-to-eat Cereals)
Experiment 4: Qualitative Detection of Lipids Using Sudan Dye Test
Part 2: Quantitative Detection of Macromolecules and Independent Investigation
Experiment 5: Quantitative Detection of Proteins Using Bradford Method Principle
Experiment 6: Qualitative Detection of Macromolecules in Various Food Items
Module 2: Cells and Membranes
Part 1: Cells, Membranes, and Diffusion
Experiment 1: Diffusion Limits of Cellular Exchange
Experiment 2: Looking at Cells through a Microscope
Experiment 3: Effects of Temperature on Membrane Integrity
Part 2: Osmosis and Diffusion across Semi-Permeable Membranes
Experiment 4: Osmosis and Diffusion through an Artificial Membrane
Experiment 5: Osmosis and Diffusion through Living Plant Membranes
5.1: Potato Cells
5.2: Elodea leaf
Module 3: Metabolism
What Is Metabolism?
Part 1: What Are Enzymes?
How Do Enzymes Catalyze Biochemical Reactions?
What Factors Influence Enzyme Activity?
Experiment 1: Investigation of Substrate Specificity of Peroxidase
Experiment 2: Effect of Environmental Conditions on the Activity of the Enzyme
2.1: Effect of Temperature on the Activity of Peroxidase
2.2: Effect of pH on the Activity of Peroxidase
Experiment 3: Effect of Inhibitors on Enzyme Activity
Part 2: Cellular Respiration
Experiment 4: Physical Activity and CO2 production
Experiment 5: Cellular Respiration in Germinating Seeds
Part 3: Photosynthesis
Experiment 6: Dependence of CO2 Uptake on Light Wavelength
Experiment 7: Balance of Photosynthesis and Cellular Respiration
7.1: Photosynthesis and Cellular Respiration in Spinach Leaves
7.2: Observations of Seedlings Grown in Light vs. Dark Conditions
Module 4–5: Molecular Biology and Mendelian Genetics
What Is DNA?
The Genetics and History of Sickle Cell Disease
Blood Types
Part 1: Molecular Genetics of Sickle Cell Disease and Blood Types
Experiment 1: Restriction Digestion of DNA Samples
Experiment 2: Agarose Gel Electrophoresis
Experiment 3: Observation of Sickle Blood Cells
Experiment 4: Blood Typing
Part 2: Gene Expression and Mutation
Experiment 5: Transcription and Translation of the Beta-Globin Gene
Experiment 6: Sickle Cell Mutation and Its Phenotype
Part 3: Mendelian Genetics and Genetic Counseling
Experiment 7: Mendel’s Law of Segregation and Chi Square
Chi Square Analysis
Experiment 8: Pedigree Analysis and Genetic Counseling
Case Study
Ethics Discussion—Application
Appendix 1: Scientific Measurement
Metric Units
Metric Conversions
Procedure 1.1: Converting Metric Units
Length
Procedure 1.2: Measuring Length
Volume
Procedure 1.3: Measuring Volume by Displacement
Mass
Procedure 1.4: Measuring Mass
Of a Solid Object
Of a Volume of Water
Temperature
Procedure 1.5: Measuring Temperature
Summary of Terms
Appendix 2: Introduction to Microscopes and Their Proper Use
Introduction
Procedure 1
Procedure 2
Basic Terminology to Know
Focusing on a Specimen
Concept Application
Appendix 3: The Laboratory Report
Introduction
The Layout
The Title
The Abstract
The Introduction Section
The Material and Method Section
The Result Section
The Discussion/Conclusion Section
The Reference Section
Citation of References in the Text (Body of the Paper)
Citation of References in the Literature Cited Section