Biology 185 Laboratory Manual & Student Study Guide offers a comprehensive list of laboratory experiments for a 100 level microbiology course, as well as, various supplemental laboratory experiments that cover core concepts and techniques for a microbiology lab. The theme of the laboratories is to develop the students understanding of the basic identification of an unknown microorganism through staining and chemical/enzymatic tests, as well as covering growth, isolation, contamination, and control of microorganisms.
The laboratory manual also contains some helpful information and tables involving microbiology concepts that can be used as a reference for course material. These concepts include history, cell structure, metabolism, chemistry, antibiotics, control of microbial growth, and immunology and are intended as a quick reference of study material for microbiology.
LABORATORY EXERCISES
LABORATORY #1 – The Microscope
LABORATORY #2 – Microscopy
LABORATORY #3 – Motile Culture
LABORATORY #4 – Simple Stain
LABORATORY #5 – Differential Stain
LABORATORY #6 – Media, Cultures, Aseptic Techniques
LABORATORY #7 – Environmental and Changing Incubation Environment
LABORATORY #8 – Beginning Enzyme Profiles
LABORATORY #9 – Clinical Microbiology and Hand Washing
LABORATORY #10 – Food and Water Microbiology
LABORATORY #11 – Chemical Methods of Microbiological Control
LABORATORY #12 – Unknown Bacterial Identification
LABORATORY SUPPLEMENTAL EXERCISES
LABORATORY #1A – Genetics Transformation Lab #1
LABORATORY #1B – Genetics Transformation Lab #2
LABORATORY #1C – Dental Caries Lab
LABORATORY #1D – Hand Washing Experiment Using Glo-Germ
LABORATORY #1E – Hand Washing Experiment
LABORATORY #1F – Fermentation Lab-Yogurt
LABORATORY #1G – Fermentation Lab-Sauerkraut
LABORATORY #1H – Fermentation Lab-Root Beer/Birch Beer
LABORATORY #1I – Fermentation Lab-Kombucha Tea
LABORATORY #1J – Fermentation Lab-Pickles
LABORATORY #1K – Heterotrophic Plate Counting
LABORATORY #1L – Transmission of Microbes
LABORATORY #1M – Antibiotic Susceptibility with Different Strains
LABORATORY #1N – Toilet paper as a filter
MICROBIOLOGY FIELD TRIPS
LANCASTER CEMETERY
LANCASTER BREWING COMPANY
STUDY GUIDE
Brief reference of important concepts/terms in microbiology
BIBLIOGRAPHY