Biology of Cells Laboratory Manual
Edition: 2
Copyright: 2014
Pages: 42
Edition: 2
Copyright: 2014
Pages: 42
Choose Your Format
$70.64 USD
Ebook
$30.51 USD
This lab manual introduces students to core techniques in molecular and cellular biology through hands-on experiments involving cell structure, molecular cloning, and protein expression and analysis. Students investigate eukaryotic cell organization, isolate and manipulate bacterial DNA, perform bacterial transformation, analyze plasmids, and examine protein expression using techniques such as SDS-PAGE and Western blotting. The manual also includes foundational laboratory skills, including micropipetting, spectrophotometry, and experimental materials preparation.
Section I: Structural Biology
Internal Organization of the Eukaryotic Cell
Section II: Molecular Cloning
Isolation and Restriction of Bacterial Genomic DNA
Transformation of Bacterial Cells with Plasmid DNA
Isolation and Restriction of Bacterial Plasmid DNA
Section III: Protein Expression and Analysis
Glutathione S-transferase: Expression and Purification
SDS-PAGE
Western Blot Detection of Glutathione S-transferase
Appendix I: Micropipettors (or Micropipets)
Appendix II: Spectrophotometry
Appendix III: Materials List
Dr. Whitney Hable is Professor Emerita of Biology. She earned a Ph.D. in Biology from the University of Arizona and a B.A. in Biology from the University of Virginia. Her teaching has included cell biology, developmental biology, and graduate biology courses. Her research focuses on cell polarization, growth and development, and the role of environmental factors in biological processes, including studies of marine brown algae and fertilization in the American eel.