Cell Biology Lab Manual

Acknowledgements 

Lab Session 1 Concentrations of Solutions, Accuracy, and Precision of Data Acquisition 
                        Part 1: Concentrations of Solutions 
                        Part 2: Accuracy, and Precision of Data Acquisition; Volume Measuring Devices 
Lab Session 2 The Microscope Part 1: Types and Proper Use 
Lab Session 3 The Microscope Part 2: Cells of Living Organisms 
Lab Session 4 Enzymes I: Spectrophotometry and Partial Puri­ cation or Tyrosinase Enzyme from Potato Tubers 
Lab Session 5 Enzymes II: Effect of the Environment on Enzyme Activity 
Lab Session 6 Enzymes III Kinetic Analysis of the Tyrosinase Enzyme: KM and VMAX 
Lab Session 7 Characterization of Proteins 
                        Part 1: Protein Separation by Gel Electrophoresis 
                        Part 2: Determining Protein Concentration: Bradford Assay 
Lab Session 8 The Study of Cellular Functions 
                         Part 1: Cell Organelle Isolation 
                         Part 2: Isolation of Mitochondria from Spinach Leaves Respiration 
Lab Session 9 The Study of Cellular Functions 
                        Part 3: Isolation of Chloroplasts from Spinach Leaves: Photosynthesis - Hill Reaction 
Lab Session 10 Enzyme Linked Immunosorbent Assay (ELISA) 
                          Addendum: Enzyme Linked Immunosorbent Assay 
Lab Session 11 Fluorescence Microscopy Immunostaining 
Lab Session 12 The Hemocytometer: Determining Density and Viability of Cells in Suspension
Lab Session 13 Protein Structure Analysis Using Online Tools