Fundamentals of Microbiology Laboratory Manual

Safety Instructions

Exercise 1: The Brightfield Microscope
Exercise 2: Other Microscopes
Exercise 3: Observing Protozoa, Algae, and Cyanobacteria
Exercise 4: Observing Fungi and Yeast
Exercise 5: Observing Bacteria
Exercise 6: Aseptic Technique
Exercise 7: Smear Preparation
Exercise 8: Simple Staining
Exercise 9: The Gram Stain
Exercise10: Miscellaneous Staining
Exercise11: Culture Media Preparation
Exercise12: Streak Plate Technique and Colony Morphology
Exercise13: Specimen Transport & Ubiquity of Microorganisms
Exercise14: Hand-washing
Exercise15: Bacterial Plate Counts
Exercise16: Bacterial Growth Characteristics
Exercise17: Selected Physiological and Biochemical Characteristics (Introducing the Gram-Negative Rods)
Exercise18: Gram-Positive Cocci: Staphylococci
Exercise19: Gram-Positive Cocci: Streptococci
Exercise20: Urine Cultures
Exercise21: Antiseptics and Disinfectants
Exercise22: Antibiotic Sensitivity Testing
Exercise23: Isolation of Antibiotic-Resistant Mutants
Exercise24: Unknowns
Exercise 25: Immunology
Exercise26: Water Microbiology
Exercise27: Spoilage of Meat
Exercise 28: Microbiology of Wine-making
Exercise29: Microbiology of Milk
Exercise 30: Isolation of an Antibiotic Producer from Soil

Appendix A: Troubleshooting the Microscope
Appendix B: Troubleshooting the Gram Stain
Appendix C: Dilution Problems
Appendix D: Flowcharts
Appendix E: Unknown Identification Table: Gram-Positive Cocci - Catalase Positive
Appendix F: Unknown Identification Table: Gram-Positive Cocci - Catalase Negative
Appendix G: Unknown Identification Table: Gram-Positive Rods
Appendix H: Unknown Identification Table: Gram-Negative Rods
Appendix I: Writing Scientific Lab Reports
Appendix J: Selected Media and Reagent Descriptions
Appendix K: Antibiotic Zone Diameter Interpretive Table
Appendix L: Spectrophotometer (Spec 20)
Appendix M: Pipetting
Appendix N: Case Histories
Case History #1
Case History #2
Case History #3
Case History #4
Case History #5
Case History #6
Appendix O: Selected References

INDEX