General Microbiology Laboratory Manual

Preface

Safety and Laboratory Guidelines

Safety Quiz

Exercise 1: Microorganisms Isolated from Natural Sources

Exercise 2: Control of Microorganisms – Effect of Hand Washing

Exercise 3: Cultural Characteristics of Microorganisms

Exercise 4: Staining of Microorganisms and Use of the Microscope

Exercise 5: The Gram Stain

Exercise 6: The Endospore Stain

Exercise 7: The Capsule Stain

Exercise 8: The Acid-fast Stain

Exercise 9: Motility

Exercise 10: Selective and Differential Media

Exercise 11: Isolation of Pure Cultures

Exercise 12: Estimation of Concentrations – Serial Dilution and Turbidity

Exercise 13: Estimation of Concentrations – Most Probable Number

Exercise 14; Microbial Growth

Exercise 15: Identification of Unknown Microbes “Pets” – Biochemical Fingerprints

Exercise 16: Environmental Effects on Growth

Exercise 17: Control of Microorganisms – Thermal Death Point

Exercise 18: Control of Microorganisms – Disinfectants

Exercise 19: Antibiotic Susceptibility/Resistance

Exercise 20: Control of Microorganisms – Ultraviolet Radiation (UV)

Exercise 21: Epidemiology – Transmission of Disease

Exercise 22; Hemolytic Activity of Microorganisms

Exercise 23: Bacteriophages and Plaque formation

Appendix