Genetics Laboratory Manual

Author(s): Chiyedza Small

Edition: 1

Copyright: 2019

Pages: 170

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Ebook

$38.20

ISBN 9781524993047

Details Electronic Delivery EBOOK 180 days

Genetics Laboratory Manual provides practical experiences for students to support the theoretical and conceptual genetics topics taught in the lecture. Students will conduct experiments that examine cell division in plant and animal cells, crossing over in Sordaria, and chromosome mapping. Using the well-studied model organism Drosophila, students will replicate Mendel's dihybrid cross to demonstrate inheritance and the law of independent assortment. Students will also examine X-linkage inheritance by using fruit flies. Several molecular genetics concepts will also be demonstrated by the extraction and analyses of DNA from animal cells as well as analysis of inheritance by studying human chromosomes.

INTRODUCTION TO BIOLOGY LABORATORY SAFETY RULES

EXERCISE 1
MITOSIS

EXERCISE 2
MEIOSIS

EXERCISE 3
MAIZE EXPERIMENTS IN GENETICS: MONOHYBRID AND DIHYBRID CROSSES AND CHI–SQUARE TEST

EXERCISE 4
INTRODUCTION TO DROSOPHILA GENETICS: X-LINKAGE

EXERCISE 5
INTRODUCTION TO DROSOPHILA GENETICS: DIHYBRID CROSS

EXERCISE 6
CROSSING OVER AND LINKAGE MAPPING

EXERCISE 7
GENETICS AND HUMAN VARIATION

EXERCISE 8
HUMAN CHROMOSOMES AND KARYOTYPES

EXERCISE 9
GENE INTERACTION AND EPISTASIS

EXERCISE 10
PEDIGREE ANALYSIS

EXERCISE 11
ISOLATION OF GENOMIC DNA FROM WHEAT GERM

EXERCISE 12
REPLICATION, TRANSCRIPTION, AND TRANSLATION

EXERCISE 13
WRITING THE LABORATORY REPORT

Chiyedza Small

Genetics Laboratory Manual provides practical experiences for students to support the theoretical and conceptual genetics topics taught in the lecture. Students will conduct experiments that examine cell division in plant and animal cells, crossing over in Sordaria, and chromosome mapping. Using the well-studied model organism Drosophila, students will replicate Mendel's dihybrid cross to demonstrate inheritance and the law of independent assortment. Students will also examine X-linkage inheritance by using fruit flies. Several molecular genetics concepts will also be demonstrated by the extraction and analyses of DNA from animal cells as well as analysis of inheritance by studying human chromosomes.

INTRODUCTION TO BIOLOGY LABORATORY SAFETY RULES

EXERCISE 1
MITOSIS

EXERCISE 2
MEIOSIS

EXERCISE 3
MAIZE EXPERIMENTS IN GENETICS: MONOHYBRID AND DIHYBRID CROSSES AND CHI–SQUARE TEST

EXERCISE 4
INTRODUCTION TO DROSOPHILA GENETICS: X-LINKAGE

EXERCISE 5
INTRODUCTION TO DROSOPHILA GENETICS: DIHYBRID CROSS

EXERCISE 6
CROSSING OVER AND LINKAGE MAPPING

EXERCISE 7
GENETICS AND HUMAN VARIATION

EXERCISE 8
HUMAN CHROMOSOMES AND KARYOTYPES

EXERCISE 9
GENE INTERACTION AND EPISTASIS

EXERCISE 10
PEDIGREE ANALYSIS

EXERCISE 11
ISOLATION OF GENOMIC DNA FROM WHEAT GERM

EXERCISE 12
REPLICATION, TRANSCRIPTION, AND TRANSLATION

EXERCISE 13
WRITING THE LABORATORY REPORT

Chiyedza Small