Genetics Laboratory Manual provides practical experiences for students to support the theoretical and conceptual genetics topics taught in the lecture. Students will conduct experiments that examine cell division in plant and animal cells, crossing over in Sordaria, and chromosome mapping. Using the well-studied model organism Drosophila, students will replicate Mendel's dihybrid cross to demonstrate inheritance and the law of independent assortment. Students will also examine X-linkage inheritance by using fruit flies. Several molecular genetics concepts will also be demonstrated by the extraction and analyses of DNA from animal cells as well as analysis of inheritance by studying human chromosomes.
INTRODUCTION TO BIOLOGY LABORATORY SAFETY RULES
EXERCISE 1
MITOSIS
EXERCISE 2
MEIOSIS
EXERCISE 3
MAIZE EXPERIMENTS IN GENETICS: MONOHYBRID AND DIHYBRID CROSSES AND CHI–SQUARE TEST
EXERCISE 4
INTRODUCTION TO DROSOPHILA GENETICS: X-LINKAGE
EXERCISE 5
INTRODUCTION TO DROSOPHILA GENETICS: DIHYBRID CROSS
EXERCISE 6
CROSSING OVER AND LINKAGE MAPPING
EXERCISE 7
GENETICS AND HUMAN VARIATION
EXERCISE 8
HUMAN CHROMOSOMES AND KARYOTYPES
EXERCISE 9
GENE INTERACTION AND EPISTASIS
EXERCISE 10
PEDIGREE ANALYSIS
EXERCISE 11
ISOLATION OF GENOMIC DNA FROM WHEAT GERM
EXERCISE 12
REPLICATION, TRANSCRIPTION, AND TRANSLATION
EXERCISE 13
WRITING THE LABORATORY REPORT