Introduction to Biotechnology II Lab Manual
Edition: 1
Copyright: 2012
Pages: 314
Edition: 1
Copyright: 2012
Pages: 314
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In A Dynamic Natural Approach to Instruction, Biotechnology II: The Fusion of Imagination, and Inquiry, students move on to more in-depth understanding of the Biology and research behind the Biotechnology industry. Biomanufactoring, DNA sequencing, plasmid mapping, protein "fingerprinting," and much more prepares students to participate in novel research annotating the genome of an important bacterium.
Each lab is written in student friendly language with essential vocabulary assignments and comprehension questions included. Students will be successful in this very hands-on program of study.
Lab 1: Reviewing Scienti?c Literature
Lab 2: Bioethics
Lab 3: Exploring Plant Structure and Function Using Wisconsin Fast Plants©
Lab 4: Global Buckets: Two Buckets on a Mission
Lab 5: Plant Tissue Culture Techniques
Lab 6: Naturopathic Botanical Medicine: Making a Healing Salve
Lab 7: Bioprocessing with Bioreactors
Lab 8: Primer Design
Lab 9: Do You Have a PV92 Insert?
Lab 10: Mitochondrial DNA Control Region Sequence and Comparison
Lab 11: DNA Sequencing
Lab 12: Bioinformatics
Lab 13: Eukaryotic Culture Cell Counts
Lab 14: Caenorhabditis elegans: Observing, Culturing, and Caring for a Model Organism
Lab 15: Plasmid Mapping
Lab 16: Determining Unknown Protein Concentration Using the Bradford Protein Assay
Lab 17a: Protein Fingerprinting
Lab 17b: Western Blotting
Lab 18: Bacterial Titration
Lab 19: Determining the Concentration of Viruses
Lab 20: What Is an Auxotroph?
Lab 21: Biotechnology Lab Skills Challenge
Lab 22: Microbial Genomics: Complementation to Test Annotation of a Gene as Important for Proline Biosynthesis
Appendix A: Microsoft Excel® in Science Class: Tables and Graphing Review
Appendix B: Bradford Assay Using the iMark™ Microplate Reader
Appendix C: Biotechnology Lab Format Review