Introduction to Forensic Biology: Basics, Lessons and Applications

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Copyright: 2026

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This textbook is designed for students with no prior science background who are fascinated by forensics. It introduces foundational biology concepts, starting with the essentials like chemistry, properties of water, organic molecules, and cell structure, then gradually builds into body systems and organisms such as bacteria, fungi, and animals that are critical in forensic investigations. Basic concepts covered in the biology foundation chapters are then applied to real-world forensic techniques, including blood spatter analysis, fingerprinting, DNA profiling, tissue decomposition, osteology, and entomology. By blending biology fundamentals with practical forensic applications, this book transforms complex science into an engaging, accessible journey for beginners eager to explore the biology behind solving crimes.

Chapter 1 The History of Forensics
Introduction 
Key Characters in the History of Forensic Science 
Conclusion

Chapter 2 Introduction to Biology 
Introduction 
Seven Aspects of Life 
       Order
       Energy Processing 
       Growth and Development
       Response to Environment
       Reproduction 
       Regulation
       Evolutionary Adaptation
Levels of Biological Organization
The Cell as the Smallest Unit of Life 
Scientific Method 
       Observations
       Hypothesis Testing
       Controls in Scientific Experimentation 
Scientific Theory 
Conclusion

Chapter 3 Introduction to Cells
Introduction 
Types of Cells
Common Features of All Cells
       Plasma Membrane 
       Ribosomes 
       Deoxyribonucleic Acid 
       Cytosol and Cytoplasm
Prokaryotic Cells
       Bacterial Cell Wall
       Glycocalyx
       Fimbriae and Pili 
Eukaryotic Cells
       Nucleus
       Rough and Smooth Endoplasmic Reticulum
       Golgi Apparatus
       Mitochondria and Chloroplasts 
       Vacuoles, Vesicles, Lysosomes, and Peroxisomes
       Cytoskeleton 
       Centrosomes and Basal Bodies
       Flagella and Cilia
       Eukaryotic Cell Wall
       Extracellular Matrix
       Cell Junctions 
Conclusion

Chapter 4 Atoms, Bonding, and the Properties of Water 
Introduction 
Introduction to Atoms
Introduction to Covalent, Ionic, and Hydrogen Bonds
       Covalent Bonding 
       Ionic Bonding 
       Polar Covalent Bonding
       Hydrogen Bonding 
Introduction to the Properties of Water
Conclusion

Chapter 5 Introduction to Macromolecules, DNA Structure and Function
Introduction 
Types of Macromolecules
       Overview
       Carbohydrates
       Lipids
       Proteins
       Nucleic Acids
DNA and RNA Structure and Function
       DNA Structure and Function 
       Ribonucleic Acid Structure and Function
Chromatin and Chromosomes
       Purpose of Chromatin
       Chromosome Structure and Function 
       Chromosome Numbers and Ploidy
       Autosomes and Sex Chromosomes
Extranuclear DNA 
       Endosymbiotic Theory
       Mitochondrial DNA
       Chloroplast DNA
       Plasmids
Conclusion

Chapter 6 Tissue Organization and Organ Systems
Introduction to the Four Tissue Types
Characteristic and Function of the Four Tissue Types
       Epithelial Tissues
       Characteristics of All Epithelial Tissues
       Nine Types of Epithelial Tissues 
       Connective Tissue
       Muscle Tissue 
       Neural Tissue 
Introduction to the Types and Function of Organ Systems 
       Integumentary System 
       Cardiovascular System 
       Respiratory System
       Skeletal System
       Endocrine System
       Muscular System 
       Digestive System 
       Lymphatic System 
       Nervous System
       Urinary System
       Reproductive System
Conclusion

Chapter 7 Changes to Soft Tissues Upon Death 
The Stages of Decomposition
Four Stages of Fresh Decay (First 3 Days)
       Pallor Mortis
       Algor Mortis
       Livor Mortis
       Rigor Mortis 
Bloat Stage (2–6 Days)
Active Decay (5–11 Days)
Advanced Decay (10–24 Days)
Dry Remains Stage (24 or More Days)
Unique Decay Conditions
       Adipocere
       Mummification 
Conditions that Affect the Stage and Rate of Decomposition 
       Environmental Conditions
       Sunlight
       Containers and Wrappings
       Water or Ground Burial 
       Wounds and Infection
       Chemical Exposure
       Burning of Remains
Conclusion

Chapter 8 Fingerprints, Hair, and Their Use for Identification
Introduction to Fingerprints
Fingerprint Patterns 
       Loop Patterns
       Whorl Patterns
       Arch Patterns 
Analyzing Fingerprints
       Analysis
       Comparison
       Evaluation
       Verification 
Collecting Fingerprints
Introduction to Hair
       Anatomy and Growth of Hair
       Transfer of Hair 
       Detection of Toxins in the Hair
Impressions of Other Objects
Conclusion

Chapter 9 Introduction to Blood and Detection Methods
Introduction 
Blood Composition and Cell Types
       Whole Blood and Its Components
       Red Blood Cells
       White Blood Cells 
Introduction to Blood Typing 
       Important Components
       Blood Type Genotypes
       Rhesus Factor
       Additional Blood Antigens
Introduction to Blood Detection Methods
       Luminol Test
       Kastle–Meyer Test 
Conclusion

Chapter 10 Bloodstain Patterns and Analysis 
Important Terminology When Examining Bloodstains
The Three Bloodstain Categories
Properties of Blood as it Relates to a Bloodstain Pattern
Introduction to Pattern from a Straight Blood Drop
Anatomy of a Bloodstain
Introduction to Passive Stain Patterns and Drip Trails
       Passive Drip Trails
       Flow Pattern
       Pools and Saturation Stains 
       Transfer Marks
Introduction to Blood Spatter
       Secondary Mechanism Spatter 
       Impact Spatter
       Cast-Off and Projection Blood Spatter
Conclusion

Chapter 11 Bone Anatomy and Composition 
Introduction to the Function of Bones
       Levers
       Protection
       Storage 
       Blood Cell Production 
Introduction to the Composition of Bones 
       Anatomy of Bone Tissue 
Introduction to Bone Cells
Classification of Bones by Structural Class and Shape
Bone Healing and Lasting Evidence of Healing 
Conclusion

Chapter 12 Anatomical Identification of Bones Including Race, Sex, and Age
Introduction 
Directional Terminology
Major Bony Landmarks Found Common to All Bones
Introduction and Landmarks in the Axial Skeleton
       The Skull
       Vertebral Column
       Thoracic Cage
Introduction and Landmarks of the Appendicular Skeleton
       Pectoral Girdle
       Humerus
       Ulna 
       Radius
       Carpals
       Metacarpals
       Phalanges
       Pelvic Girdle 
       Lower Limb of the Body 
Distinguishing Sex Based on Bone Differences
Distinguishing Age Based on Differences in the Bones
Conclusion

Chapter 13 Wounds: Patterns and Mechanism to Tissues 
Introduction to Kinetic Wounds and Non-Kinetic Wounds
       Blunt Force Wounds 
Introduction to Sharp Force Traumas
       Incised Wounds
       Stab Wounds
Introduction to Asphyxia 
       Mechanism of Action
       Signs of Asphyxia
       Positional Asphyxia 
Introduction to Firearm Wounds
       Types of Firearms and Ammunition 
       Factors Affecting Firearm Wound Appearance
       Bullet Wounds in Bones 
Introduction to Bite Marks 
Introduction to Burns
Introduction to Defense Wounds
Conclusion

Chapter 14 Urine, Feces, Saliva, and Semen: Identification and Detection 
Introduction to Urine and Feces
       Liver Detoxification and Production of Urine and Feces
       Feces Analysis
       Urine Analysis 
Introduction to Saliva 
       Salivary Gland Function and Location 
       Saliva and Secreter Status
Introduction to Semen 
       Components and Production of Semen 
       Techniques for Isolating Semen Cells 
Conclusion

Chapter 15 DNA Replication 
Introduction 
Enzymes Are Key Proteins in the Cell 
       Enzyme Components
       Enzyme Environmental Factors
DNA Replication Initiation
       Origin of Replication and Replication Forks
DNA Replication Extension 
       Single-Stranded Binding Proteins 
       Primase and DNA Polymerase III 
       Directionality of Deoxyribonucleic Acid 
       Directionality of DNA Polymerase
       Leading and Lagging Strands
       DNA Polymerase I and Ligase 
Telomeres and Telomerase Activity
Conclusion

Chapter 16 Transcription and Translation 
Introduction 
The Role of RNA in Gene Expression 
       RNA Structure and Types
Gene Expression Components and Overview 
       Gene Expression in Prokaryotes and Eukaryotes
       Triplet Codons and the Genetic Code
       Directionality of Gene Expression 
       The Genetic Code Is Redundant and Universal
Transcription: Copying the DNA into RNA
       Initiation of Transcription
       Elongation of Transcription 
       Termination of Transcription 
       Eukaryotic RNA Processing 
       Splicing and the Spliceosome
Translation: Synthesizing the Polypeptide 
       Structure and Role of tRNA
       Ribosome Structure 
       Translation Initiation
       Translation Elongation
       Translation Termination
       Protein Folding and Function after Translation
Mutations: Alterations in the Genetic Code 
       Specific Types of Mutations
       Causes of Mutations 
Conclusion

Chapter 17 DNA Profiling in Forensics 
First Case Solved by DNA Evidence
Overview of Genetics and Inheritance 
DNA Structure and Function in Forensics
DNA Repeats: Short Tandem Repeats, Variable Number Tandem Repeats, and Satellite DNA
Short Tandem Repeats (STRs) and the Combined DNA Index System
Mutations and Single-Nucleotide Polymorphisms in Forensics
Mitochondrial DNA and Its Function in Forensics 
Conclusion

Chapter 18 Biotechnology in Forensics
Introduction 
DNA Collection and Contamination Control 
DNA Transfer and Trace Evidence
Polymerase Chain Reaction
Gel Electrophoresis
Capillary Electrophoresis 
DNA Sequencing
Wildlife DNA and DNA Barcoding
Conclusion

Chapter 19 Introduction to the Domains of Life
Introduction 
The Role of Taxonomy and Phylogeny in Organizing Life
       Classifications and the Initiation of Binomial Nomenclature
       The Science of Phylogeny 
The Theory of Evolution 
       Contributions of Charles Darwin
       Natural Selection and the Role in Evolution 
The Role of 16S rRNA in the Three-Domain System
The Three Domains of Life 
       Introduction to Domain Archaea 
       Introduction to Domain Bacteria 
       Introduction to Domain Eukarya 
Conclusion

Chapter 20 Domain Bacteria: Life and Death at the Microscopic Level
Introduction 
Main Characteristics of Bacteria 
       Shared Bacterial Characteristics
       Shapes and Arrangements of Bacteria 
       Energy Production through Cellular Respiration and Fermentation 
Bacterial Identification and Significance
The Human Microbiome and Necrobiome
       Locations of Microbiomes
       Human Microbiome Project 
       The Necrobiome 
Bacterial Agents of Bioterrorism
       Anthrax 
       Botulism
       Plague 
Conclusion

Chapter 21 Kingdom Fungi: Decomposition and Poisons 
Introduction 
Cell Maintenance: Energy Requirements and Cell Division
       Phototrophs, Chemotrophs, and Saprotrophs
       Mitosis and Meiosis
Main Characteristics of Fungi 
Important Phyla of Kingdom Fungi
       Basidiomycota 
       Ascomycota 
       Zygomycota 
Forensic Mycology: A Developing Science 
Mycotoxins
       Aflatoxin
       Ochratoxin A
       Ergot 
       Fusariotoxins 
Mushroom Poisoning
Conclusion

Chapter 22 Kingdom Animalia: Species and Application in Forensic Biology
Introduction 
An Overview of Kingdom Animalia
       General Characteristics of Kingdom Animalia
       Embryonic Development and Germ Layers 
       Symmetry and Body Plans 
Invertebrates: A Major Group of Kingdom Animalia
Characteristics of Insects
Life Cycle of Flies
Forensic Entomology in Criminal Investigations
Conclusion

Chapter 23 Careers and Future Directions in Forensic Biology
Introduction 
Careers in Forensic Biology 
       Forensic Pathologist
       Forensic Toxicologist
       Forensic Entomologist
       Forensic Odontologist
       Forensic Nurse 
       Forensic Scientist
Next Steps in Forensic Biology 
       Microbial Forensics
       Mitochondrial DNA Databases
       Use of Genetic Databases
       Epigenetics
       The Use of Artificial Intelligence 
Conclusion

Bibliography
Glossary

Tracy Lee

Tracy Lee is an associate professor of biology and the current co-chair of the department of biology at Pikes Peak State College. She received her B.A. in exercise science and teaching certificate from Metro State University in Denver, Colorado. She earned her M.S. in biology with an emphasis in anatomy and physiology from the University of Nebraska Kearney. She teaches undergraduate courses in anatomy and physiology and is responsible for the advanced cadaver dissection courses at the college. When not teaching, Professor Lee can be found riding horses or hiking in the mountains with her family and pets.

Amber Marean

Amber Marean is a professor of biology and the current co-chair of the department of biology at Pikes Peak State College. She received her B.A. in biology/chemistry from Point Loma Nazarene University in San Diego, California. She earned her Ph.D. in molecular biology from University of Colorado Anschutz in Aurora, Colorado. She is a recipient of an NSF grant, Creating Course-Based Undergraduate Research Experiences in Gene Editing for All Students, which helps bring CRISPR to community college students throughout the United States. She teaches undergraduate courses in introductory biology, microbiology, and genetics. Dr. Marean loves hiking and spending time in the mountains and visiting Disney parks with family and friends.

This textbook is designed for students with no prior science background who are fascinated by forensics. It introduces foundational biology concepts, starting with the essentials like chemistry, properties of water, organic molecules, and cell structure, then gradually builds into body systems and organisms such as bacteria, fungi, and animals that are critical in forensic investigations. Basic concepts covered in the biology foundation chapters are then applied to real-world forensic techniques, including blood spatter analysis, fingerprinting, DNA profiling, tissue decomposition, osteology, and entomology. By blending biology fundamentals with practical forensic applications, this book transforms complex science into an engaging, accessible journey for beginners eager to explore the biology behind solving crimes.

Chapter 1 The History of Forensics
Introduction 
Key Characters in the History of Forensic Science 
Conclusion

Chapter 2 Introduction to Biology 
Introduction 
Seven Aspects of Life 
       Order
       Energy Processing 
       Growth and Development
       Response to Environment
       Reproduction 
       Regulation
       Evolutionary Adaptation
Levels of Biological Organization
The Cell as the Smallest Unit of Life 
Scientific Method 
       Observations
       Hypothesis Testing
       Controls in Scientific Experimentation 
Scientific Theory 
Conclusion

Chapter 3 Introduction to Cells
Introduction 
Types of Cells
Common Features of All Cells
       Plasma Membrane 
       Ribosomes 
       Deoxyribonucleic Acid 
       Cytosol and Cytoplasm
Prokaryotic Cells
       Bacterial Cell Wall
       Glycocalyx
       Fimbriae and Pili 
Eukaryotic Cells
       Nucleus
       Rough and Smooth Endoplasmic Reticulum
       Golgi Apparatus
       Mitochondria and Chloroplasts 
       Vacuoles, Vesicles, Lysosomes, and Peroxisomes
       Cytoskeleton 
       Centrosomes and Basal Bodies
       Flagella and Cilia
       Eukaryotic Cell Wall
       Extracellular Matrix
       Cell Junctions 
Conclusion

Chapter 4 Atoms, Bonding, and the Properties of Water 
Introduction 
Introduction to Atoms
Introduction to Covalent, Ionic, and Hydrogen Bonds
       Covalent Bonding 
       Ionic Bonding 
       Polar Covalent Bonding
       Hydrogen Bonding 
Introduction to the Properties of Water
Conclusion

Chapter 5 Introduction to Macromolecules, DNA Structure and Function
Introduction 
Types of Macromolecules
       Overview
       Carbohydrates
       Lipids
       Proteins
       Nucleic Acids
DNA and RNA Structure and Function
       DNA Structure and Function 
       Ribonucleic Acid Structure and Function
Chromatin and Chromosomes
       Purpose of Chromatin
       Chromosome Structure and Function 
       Chromosome Numbers and Ploidy
       Autosomes and Sex Chromosomes
Extranuclear DNA 
       Endosymbiotic Theory
       Mitochondrial DNA
       Chloroplast DNA
       Plasmids
Conclusion

Chapter 6 Tissue Organization and Organ Systems
Introduction to the Four Tissue Types
Characteristic and Function of the Four Tissue Types
       Epithelial Tissues
       Characteristics of All Epithelial Tissues
       Nine Types of Epithelial Tissues 
       Connective Tissue
       Muscle Tissue 
       Neural Tissue 
Introduction to the Types and Function of Organ Systems 
       Integumentary System 
       Cardiovascular System 
       Respiratory System
       Skeletal System
       Endocrine System
       Muscular System 
       Digestive System 
       Lymphatic System 
       Nervous System
       Urinary System
       Reproductive System
Conclusion

Chapter 7 Changes to Soft Tissues Upon Death 
The Stages of Decomposition
Four Stages of Fresh Decay (First 3 Days)
       Pallor Mortis
       Algor Mortis
       Livor Mortis
       Rigor Mortis 
Bloat Stage (2–6 Days)
Active Decay (5–11 Days)
Advanced Decay (10–24 Days)
Dry Remains Stage (24 or More Days)
Unique Decay Conditions
       Adipocere
       Mummification 
Conditions that Affect the Stage and Rate of Decomposition 
       Environmental Conditions
       Sunlight
       Containers and Wrappings
       Water or Ground Burial 
       Wounds and Infection
       Chemical Exposure
       Burning of Remains
Conclusion

Chapter 8 Fingerprints, Hair, and Their Use for Identification
Introduction to Fingerprints
Fingerprint Patterns 
       Loop Patterns
       Whorl Patterns
       Arch Patterns 
Analyzing Fingerprints
       Analysis
       Comparison
       Evaluation
       Verification 
Collecting Fingerprints
Introduction to Hair
       Anatomy and Growth of Hair
       Transfer of Hair 
       Detection of Toxins in the Hair
Impressions of Other Objects
Conclusion

Chapter 9 Introduction to Blood and Detection Methods
Introduction 
Blood Composition and Cell Types
       Whole Blood and Its Components
       Red Blood Cells
       White Blood Cells 
Introduction to Blood Typing 
       Important Components
       Blood Type Genotypes
       Rhesus Factor
       Additional Blood Antigens
Introduction to Blood Detection Methods
       Luminol Test
       Kastle–Meyer Test 
Conclusion

Chapter 10 Bloodstain Patterns and Analysis 
Important Terminology When Examining Bloodstains
The Three Bloodstain Categories
Properties of Blood as it Relates to a Bloodstain Pattern
Introduction to Pattern from a Straight Blood Drop
Anatomy of a Bloodstain
Introduction to Passive Stain Patterns and Drip Trails
       Passive Drip Trails
       Flow Pattern
       Pools and Saturation Stains 
       Transfer Marks
Introduction to Blood Spatter
       Secondary Mechanism Spatter 
       Impact Spatter
       Cast-Off and Projection Blood Spatter
Conclusion

Chapter 11 Bone Anatomy and Composition 
Introduction to the Function of Bones
       Levers
       Protection
       Storage 
       Blood Cell Production 
Introduction to the Composition of Bones 
       Anatomy of Bone Tissue 
Introduction to Bone Cells
Classification of Bones by Structural Class and Shape
Bone Healing and Lasting Evidence of Healing 
Conclusion

Chapter 12 Anatomical Identification of Bones Including Race, Sex, and Age
Introduction 
Directional Terminology
Major Bony Landmarks Found Common to All Bones
Introduction and Landmarks in the Axial Skeleton
       The Skull
       Vertebral Column
       Thoracic Cage
Introduction and Landmarks of the Appendicular Skeleton
       Pectoral Girdle
       Humerus
       Ulna 
       Radius
       Carpals
       Metacarpals
       Phalanges
       Pelvic Girdle 
       Lower Limb of the Body 
Distinguishing Sex Based on Bone Differences
Distinguishing Age Based on Differences in the Bones
Conclusion

Chapter 13 Wounds: Patterns and Mechanism to Tissues 
Introduction to Kinetic Wounds and Non-Kinetic Wounds
       Blunt Force Wounds 
Introduction to Sharp Force Traumas
       Incised Wounds
       Stab Wounds
Introduction to Asphyxia 
       Mechanism of Action
       Signs of Asphyxia
       Positional Asphyxia 
Introduction to Firearm Wounds
       Types of Firearms and Ammunition 
       Factors Affecting Firearm Wound Appearance
       Bullet Wounds in Bones 
Introduction to Bite Marks 
Introduction to Burns
Introduction to Defense Wounds
Conclusion

Chapter 14 Urine, Feces, Saliva, and Semen: Identification and Detection 
Introduction to Urine and Feces
       Liver Detoxification and Production of Urine and Feces
       Feces Analysis
       Urine Analysis 
Introduction to Saliva 
       Salivary Gland Function and Location 
       Saliva and Secreter Status
Introduction to Semen 
       Components and Production of Semen 
       Techniques for Isolating Semen Cells 
Conclusion

Chapter 15 DNA Replication 
Introduction 
Enzymes Are Key Proteins in the Cell 
       Enzyme Components
       Enzyme Environmental Factors
DNA Replication Initiation
       Origin of Replication and Replication Forks
DNA Replication Extension 
       Single-Stranded Binding Proteins 
       Primase and DNA Polymerase III 
       Directionality of Deoxyribonucleic Acid 
       Directionality of DNA Polymerase
       Leading and Lagging Strands
       DNA Polymerase I and Ligase 
Telomeres and Telomerase Activity
Conclusion

Chapter 16 Transcription and Translation 
Introduction 
The Role of RNA in Gene Expression 
       RNA Structure and Types
Gene Expression Components and Overview 
       Gene Expression in Prokaryotes and Eukaryotes
       Triplet Codons and the Genetic Code
       Directionality of Gene Expression 
       The Genetic Code Is Redundant and Universal
Transcription: Copying the DNA into RNA
       Initiation of Transcription
       Elongation of Transcription 
       Termination of Transcription 
       Eukaryotic RNA Processing 
       Splicing and the Spliceosome
Translation: Synthesizing the Polypeptide 
       Structure and Role of tRNA
       Ribosome Structure 
       Translation Initiation
       Translation Elongation
       Translation Termination
       Protein Folding and Function after Translation
Mutations: Alterations in the Genetic Code 
       Specific Types of Mutations
       Causes of Mutations 
Conclusion

Chapter 17 DNA Profiling in Forensics 
First Case Solved by DNA Evidence
Overview of Genetics and Inheritance 
DNA Structure and Function in Forensics
DNA Repeats: Short Tandem Repeats, Variable Number Tandem Repeats, and Satellite DNA
Short Tandem Repeats (STRs) and the Combined DNA Index System
Mutations and Single-Nucleotide Polymorphisms in Forensics
Mitochondrial DNA and Its Function in Forensics 
Conclusion

Chapter 18 Biotechnology in Forensics
Introduction 
DNA Collection and Contamination Control 
DNA Transfer and Trace Evidence
Polymerase Chain Reaction
Gel Electrophoresis
Capillary Electrophoresis 
DNA Sequencing
Wildlife DNA and DNA Barcoding
Conclusion

Chapter 19 Introduction to the Domains of Life
Introduction 
The Role of Taxonomy and Phylogeny in Organizing Life
       Classifications and the Initiation of Binomial Nomenclature
       The Science of Phylogeny 
The Theory of Evolution 
       Contributions of Charles Darwin
       Natural Selection and the Role in Evolution 
The Role of 16S rRNA in the Three-Domain System
The Three Domains of Life 
       Introduction to Domain Archaea 
       Introduction to Domain Bacteria 
       Introduction to Domain Eukarya 
Conclusion

Chapter 20 Domain Bacteria: Life and Death at the Microscopic Level
Introduction 
Main Characteristics of Bacteria 
       Shared Bacterial Characteristics
       Shapes and Arrangements of Bacteria 
       Energy Production through Cellular Respiration and Fermentation 
Bacterial Identification and Significance
The Human Microbiome and Necrobiome
       Locations of Microbiomes
       Human Microbiome Project 
       The Necrobiome 
Bacterial Agents of Bioterrorism
       Anthrax 
       Botulism
       Plague 
Conclusion

Chapter 21 Kingdom Fungi: Decomposition and Poisons 
Introduction 
Cell Maintenance: Energy Requirements and Cell Division
       Phototrophs, Chemotrophs, and Saprotrophs
       Mitosis and Meiosis
Main Characteristics of Fungi 
Important Phyla of Kingdom Fungi
       Basidiomycota 
       Ascomycota 
       Zygomycota 
Forensic Mycology: A Developing Science 
Mycotoxins
       Aflatoxin
       Ochratoxin A
       Ergot 
       Fusariotoxins 
Mushroom Poisoning
Conclusion

Chapter 22 Kingdom Animalia: Species and Application in Forensic Biology
Introduction 
An Overview of Kingdom Animalia
       General Characteristics of Kingdom Animalia
       Embryonic Development and Germ Layers 
       Symmetry and Body Plans 
Invertebrates: A Major Group of Kingdom Animalia
Characteristics of Insects
Life Cycle of Flies
Forensic Entomology in Criminal Investigations
Conclusion

Chapter 23 Careers and Future Directions in Forensic Biology
Introduction 
Careers in Forensic Biology 
       Forensic Pathologist
       Forensic Toxicologist
       Forensic Entomologist
       Forensic Odontologist
       Forensic Nurse 
       Forensic Scientist
Next Steps in Forensic Biology 
       Microbial Forensics
       Mitochondrial DNA Databases
       Use of Genetic Databases
       Epigenetics
       The Use of Artificial Intelligence 
Conclusion

Bibliography
Glossary

Tracy Lee

Tracy Lee is an associate professor of biology and the current co-chair of the department of biology at Pikes Peak State College. She received her B.A. in exercise science and teaching certificate from Metro State University in Denver, Colorado. She earned her M.S. in biology with an emphasis in anatomy and physiology from the University of Nebraska Kearney. She teaches undergraduate courses in anatomy and physiology and is responsible for the advanced cadaver dissection courses at the college. When not teaching, Professor Lee can be found riding horses or hiking in the mountains with her family and pets.

Amber Marean

Amber Marean is a professor of biology and the current co-chair of the department of biology at Pikes Peak State College. She received her B.A. in biology/chemistry from Point Loma Nazarene University in San Diego, California. She earned her Ph.D. in molecular biology from University of Colorado Anschutz in Aurora, Colorado. She is a recipient of an NSF grant, Creating Course-Based Undergraduate Research Experiences in Gene Editing for All Students, which helps bring CRISPR to community college students throughout the United States. She teaches undergraduate courses in introductory biology, microbiology, and genetics. Dr. Marean loves hiking and spending time in the mountains and visiting Disney parks with family and friends.