Scientists have demonstrated their ability to offer at least some hope of survival to those facing serious medical diagnoses. Scientists are far from the heartless analytical machines portrayed in modern literature, and doctors are much more rational than the actors pretending to emote all over their patients on certain TV shows. Scientists and physicians are people who have, by and large, taken an oath to let Nature be their primary teacher so that they can, in one way or other, help other humans and humanity thrive. Every diagnostic or treatment procedure in medicine has either been tested and shown to be effective, been likewise demonstrated ineffective or awaits careful scrutiny. This testing requires science.
This lab manual guides students through this learning and discovery process of biological health science, both imparting information and prompting observation simultaneously.
Preface
The Relation Between Science and Medicine
0.1 Introduction
0.2 Science is the basis of modern medicine
0.3 The scientific method
0.4 Exercise
1 Laboratory Techniques
1.1 Introduction
1.2 Metric madness
1.2.1 Length
1.2.2 Volume
1.2.3 Mass
1.2.4 Temperature
1.3 Significant figures
1.4 Solutions
1.5 Exercises
2 Measurement, Data, and Experiments
2.1 Introduction
2.1.1 Types of studies
2.1.2 Controls, masking, and separation of variables
2.1.3 Enzymes
2.2 Does catalase exist in the mammalian liver?
2.3 How does catalase respond to changes in pH?
2.3.1 Acids and bases
2.3.2 Experimental procedure
2.4 Exercises
3 Atoms and Biomolecules
3.1 Introduction
3.2 Properties of atoms
3.3 Molecules and macromolecules
3.4 Exercises
4 Eat, Drink, and Feel Guilty
4.1 The tragic diet of Joe College
4.2 Personal diet analysis
4.3 Exercises
5 Cells and Tissues
5.1 Introduction
5.2 Proper microscopy technique
5.3 Prokaryotic cells
5.4 Eukaryotic cells
5.5 Histology
5.5.1 Epithelial tissue
5.5.2 Connective tissue
5.5.3 Muscle tissue
5.5.4 Nervous tissue
5.6 Exercises
6 Cellular Respiration
6.1 Introduction
6.2 Cellular respiration
6.3 Molecular models
6.4 Activity and CO2 production
6.5 Exercises
7 Mitosis and Meiosis
7.1 Introduction
7.2 Mitosis using bead models
7.3 Meiosis using bead models
Meiosis I:
Meiosis II:
7.4 Mitosis in the whitefish blastula
7.5 Exercises
8 Genetics
8.1 Introduction
8.2 Testing Mendel’s Law of Segregation
8.2.1 Calculating x2
8.2.2 Interpreting x2
8.2.3 Testing Mendel’s first law in corn
8.3 Testing Mendel’s Law of Independent Assortment
8.4 Karyotype Analysis
8.5 Exercises
9 DNA Profiling
9.1 Outbreak
9.2 DNA fingerprinting
9.3 Isolating DNA
9.4 DNA fingerprinting theory
9.5 Exercises
10 Gene Action
10.1 Introduction
10.2 Transcription
10.3 Translation
10.4 Exercises
11 Epidemiology
11.1 Introduction
11.2 The two faces of medicine
11.3 The challenge
11.4 Exercises
12 Natural Selection
12.1 Introduction
12.2 Natural selection simulation
12.3 Exercises