Investigative Microbiology Lab Manual: Isolation and Characterization of Soil Bacteria

Acknowledgments 
About the Author
Introduction 
Lab Safety

Section 1 Microscopy

Section 2 Sterilization

Section 3 Aseptic Technique

Section 4 Isolation of Pure Cultures
Paper 1
Paper 2

Section 5 Soil Collection and Sampling

Section 6 Screening for Antibiotic Production

Section 7 Culture Media
7.1 Mannitol Salt Agar (MSA)—Selective and Differential
7.2 MacConkey Agar—Selective and Differential
7.3 Eosin Methylene Blue (EMB) Agar—Selective and Differential
7.4 Phenylethyl Alcohol Agar (PEA Agar)—Selective
7.5 Blood Agar—Enriched and Differential
7.6 Thioglycolate Medium—Oxygen Requirements with differential

Section 8 Molecular Techniques 
8.1 DNA Extraction 
8.2 Polymerase Chain Reaction (PCR)
8.3 Agarose Gel Electrophoresis
8.4 Sanger Sequencing and BLAST Analysis of 16S rRNA Genes 

Section 9 Staining
9.1 Smear Preparation
9.2 Gram Staining 
9.3 Endospore Staining (Schaeffer–Fulton Method) 

Section 10 Physical and Chemical Controls of Bacterial Growth
10.1 Physical Methods of Control 
10.2 Chemical Control Methods

Section 11 Antibiotics and Antimicrobials

Section 12 Biochemical Assays 
12.1 Extracellular Enzymes
          Starch Hydrolysis
          Urea Hydrolysis
          Gelatin Hydrolysis
12.2 Carbohydrate Fermentation 
          Oxidase Test
          Catalase Test 
          Nitrate Reduction Test 
12.4 IMViC Tests
          Indole Test—SIM Agar
          Methyl Red (MR) and Voges–Proskauer (VP) Tests
          Citrate Utilization Test