JAMM Lab Manual for Microbiology

Features and Resources for Students
Acknowledgments
Unit 1 Microscopy and Eukaryotic Microbes
 Introduction to Safety and Handwashing 
Exercise 1 Lab Safety and General Microbiology Procedures
Exercise 2 Effective Handwashing
Introduction to Microscopy
Exercise 3 Bright Field Microscopy
Introduction to Eukaryotic Microbes
Exercise 4 Protists and Pond Water
Exercise 5 Fungi of Medical and Economic Importance
Exercise 6 Protozoans of Medical Importance
Exercise 7 Parasitic Helminths 
Unit 2 Staining and Culture Methods 
Exercise 8 Ubiquity of Microorganisms
Exercise 9 Aseptic Techniques
Introduction to Bacterial Structure and Staining 
Exercise 10 Smear Preparation and Simple Stain
Exercise 11 Negative Stain 
Introduction to Differential Stains 
Exercise 12 Gram Stain
Exercise 13 Acid-Fast Stains
Introduction to Structural Stains
Exercise 14 Endospore Stain
Exercise 15 Capsule Stain 
Exercise 16 Motility and Flagella Arrangements 
Introduction to Environmental Factors Affecting Microbial Growth....115 
Exercise 17 Pure Culture Techniques 
Exercise 18 Effect of Nutrients on Bacterial Growth
Exercise 19 Effect of Temperature on Bacterial Growth 
Exercise 20 Effect of Oxygen on Bacterial Growth
Exercise 21 Effect of Ultraviolet Radiation on Bacterial Growth 
Exercise 22 Bacterial Counts on Food
Exercise 23 Bacteriological Examination of Water: Most Probable Number
Exercise 24 Fermentation in Food Production 
Exercise 25 Antimicrobial Susceptibility Test
Exercise 26 Evaluation of Antiseptics and Disinfectants 
Introduction to Acellular Microbes 
Exercise 27 Phage Titer
Exercise 28 Phage Typing 
Unit 3 Identification of an Unknown and Medical Studies 
Exercise 29 Stock Cultures and Bacterial Morphology  
Introduction to Cultural Characteristics  
Exercise 30 Colony Morphology 
Exercise 31 Growth Patterns in Broth 
Exercise 32 Growth Patterns on Slants  
Introduction to Respiration Tests 
Exercise 33 Oxygen Requirements Using FTM 
Exercise 34 Catalase Test 
Exercise 35 Oxidase Test  
Exercise 36 Nitrate Reduction Test
Introduction to Fermentation Tests 
Exercise 37 Fermentation of Sugars Using Phenol Red Broth 
Exercise 38 Methyl Red and Voges–Proskauer Tests  
Introduction to Hydrolysis Tests 
Exercise 39 Starch Hydrolysis Test  
Exercise 40 Casein Hydrolysis Test  
Exercise 41 Lipid Hydrolysis Test   
Exercise 42 Gelatin Hydrolysis Test   
Exercise 43 Urea Hydrolysis Test  
Introduction to Degradative Tests 
Exercise 44 Citrate Utilization Test  
Exercise 45 Phenylalanine Deamination Test
Exercise 46 SIM Medium: Sulfur Reduction, Indole Production, and Motility 
Introduction to Methods of Identification 
Exercise 47 Final Identification of Unknown Bacterium 
Exercise 48 Rapid Multi-Test System for Enteric Bacteria 
Introduction to Medical Microbiology  
Exercise 49 Tracking Diseases in Populations  
Exercise 50 Synthetic Epidemic Simulation 
Exercise 41 Serology: Slide Agglutination Test  
Exercise 52 Serology: Latex Agglutination Test
Appendix: List of Bacteria Used in This Manual