Laboratory Manual for Biochemistry

Author(s): Mark Sinton

Edition: 2

Copyright: 2021

Pages: 120

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$35.00

ISBN 9781792484308

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The NEW second edition of Sinton's Laboratory Manual for Biochemistry represents a complete update and revision from the first edition, including many new exercises. In response to the increased importance of NMR and food in biochemistry, for example, several new exercises have been added: amino acid pK2, determination via protein NMR, solution carbohydrate identification via carbon NMR, and explorations of food carbohydrate and fat content. And while the original catalase purification scheme and associated activity studies remain (all-be-it updated), an entirely new Russet potato tyrosinase purification scheme and activity studies have been added.  The inclusion of the second enzyme exercises will allow faculty to choose the enzyme exercises that best suit the duration of their lab period and available equipment.  In addition to the new activities, all of the art work from the first edition has been updated, as well as the appendix and faculty manual.  

LABORATORY SAFETY
LABORATORY NOTEBOOKS
SIGNIFICANT FIGURES AND MORE

EXERCISE 1 Chemical Drawing Software

EXERCISE 2 Chemical Visualization Software

EXERCISE 3 Pipettors

EXERCISE 4 Buffers

EXERCISE 5 NMR Amino Acid pKa Analysis

EXERCISE 6 Bradford Assay Analysis

EXERCISE 7 Chromatographic Analysis

EXERCISE 8 Electrophoretic Analysis

EXERCISE 9 Food Sugar Analysis

EXERCISE 10 Food Fat Analysis

EXERCISE 11 Food Energy Analysis

EXERCISE 12 Enzyme Activity Analysis

EXERCISE 13 Enzyme Kinetic Analysis

EXERCISE 14 Enzyme Purification

EXERCISE 15 Purified Enzyme Environmental Analysis

EXERCISE 16 Purified Enzyme Kinetic Analysis

EXERCISE 17 Purified Enzyme Effector Analysis

EXERCISE 18 Purified Enzyme Affinity Labeling Analysis

EXERCISE 19 Purified Enzyme Docking Analysis

APPENDIX

Mark Sinton

The NEW second edition of Sinton's Laboratory Manual for Biochemistry represents a complete update and revision from the first edition, including many new exercises. In response to the increased importance of NMR and food in biochemistry, for example, several new exercises have been added: amino acid pK2, determination via protein NMR, solution carbohydrate identification via carbon NMR, and explorations of food carbohydrate and fat content. And while the original catalase purification scheme and associated activity studies remain (all-be-it updated), an entirely new Russet potato tyrosinase purification scheme and activity studies have been added.  The inclusion of the second enzyme exercises will allow faculty to choose the enzyme exercises that best suit the duration of their lab period and available equipment.  In addition to the new activities, all of the art work from the first edition has been updated, as well as the appendix and faculty manual.  

LABORATORY SAFETY
LABORATORY NOTEBOOKS
SIGNIFICANT FIGURES AND MORE

EXERCISE 1 Chemical Drawing Software

EXERCISE 2 Chemical Visualization Software

EXERCISE 3 Pipettors

EXERCISE 4 Buffers

EXERCISE 5 NMR Amino Acid pKa Analysis

EXERCISE 6 Bradford Assay Analysis

EXERCISE 7 Chromatographic Analysis

EXERCISE 8 Electrophoretic Analysis

EXERCISE 9 Food Sugar Analysis

EXERCISE 10 Food Fat Analysis

EXERCISE 11 Food Energy Analysis

EXERCISE 12 Enzyme Activity Analysis

EXERCISE 13 Enzyme Kinetic Analysis

EXERCISE 14 Enzyme Purification

EXERCISE 15 Purified Enzyme Environmental Analysis

EXERCISE 16 Purified Enzyme Kinetic Analysis

EXERCISE 17 Purified Enzyme Effector Analysis

EXERCISE 18 Purified Enzyme Affinity Labeling Analysis

EXERCISE 19 Purified Enzyme Docking Analysis

APPENDIX

Mark Sinton