Microbiology for Health Professionals

Laboratory Safety Rules 

LAB 1: Ubiquity of Microorganisms (2-Day Lab) 
Table 1-1: Colony Characteristics 
Figure 1.1: Ubiquity of Microorganisms Plate 
Conclusions 
Questions 

LAB 2: Microscopy (3-Day Lab) 
Figure 2.1: Cryptosporidium parvum (400x prepared slide) 
Figure 2.2: Trypanosoma sp (400x prepared slide)
Figure 2.3: Aspergillus sp. 
Figure 2.4: Bacteria—Three Types (1000x prepared slide) 
Figure 2.5: Treponema pallidum (1000x prepared slide) 
Figure 2.6: Bacillus subtilis (1000x prepared slide) 
Figure 2.7: Capsule stain (Klebsiella pneumonia) (1000x prepared slide) 
Figure 2.8: Escherichia coli (1000x stained with methylene blue) 
Questions 

LAB 3: Gram Stain (2-Day Lab) 
Figure 3.1: Results of Gram stain for each species of bacteria 
Figure 3.2: Streak for isolation plate drawing 
Questions 

LAB 4: Bacterial Growth Media 
Questions 

LAB 5: Serial Dilutions (2-Day Lab) 
Figure 5.1: Serial Dilution Schematic 
Table 5-1: Serial Dilution Plate Count
Figure 5.2: Example of a Statistically Valid Plate 
Conclusions 
Questions 

LAB 6: Viral Plaques (2-Day Lab) 
Figure 6.1: Serial Dilution Schematic 
Table 6-1: Serial Dilution Plate Count
Figure 6.2: Example of a Statistically Valid Plate 
Questions 

LAB 7: Growth Curve and Viable Plate Count (2-Day Lab) 
Table 7-1: Reporting Growth Curve Data 
Figure 7.1: Graph of Bacterial Growth Curve (Time vs. CFU/mL) 
Figure 7.2: Graph of Bacterial Growth Curve (Time vs. A660) 
Figure 7.3: Graph Showing Comparison of estimates of bacterial growth (CFU/mL vs. A660)
Conclusions 
Questions 

LAB 8: UVGI (2-Day Lab) 
Table 8-1 
Results 
Figure 8.1: Mean Percent Kill as a Function of UVGI Dose 
Conclusions 
Questions 

LAB 9: Differentiation of Gram Positive Cocci (3-Day Lab) 
Table 9-1: Metabolic and Anatomic Differences between Cocci
Figure 9.1: MSA Plate 
Figure 9.2: TGA Plate 
Figure 9.3: TSA with 5% Sheep’s blood plate, also known as Sheep Blood Agar (SBA) 
Figure 9.4: Body Swab on MSA plate 
Conclusions 
Questions 

LAB 10: Antibiotic Effectiveness (2-Day Lab) 
Table 10-1: Interpretation of Zone of Inhibition of S. aureus Strains when Challenged with Selected Antibiotics 
Table 10-2: Interpretation of Zone of Inhibition of S. aureus Strains when Challenged with Selected Antibiotics 
Figure 10.1: Kirby Bauer Disk Diffusion Plates 
Conclusions 
Questions 

Lab 11: Identification of Gram Negative Bacteria (2–4 Day Lab) 
Table 11-1: Metabolic Results on Selected Enterics
Figure 11.1: Metabolic Test on Stock Enterics (Draw test tube results.) 
Figure 11.2: EMB Streak Plate of Isolated/Unknown Enteric from Fecal Sample 
Figure 11.3: TSA plate of Isolated/Unknown Enteric from Fecal Sample. 
Conclusions 
Questions 

LAB 12: Bacterial Conjugation 
Results 
Figure 12.1: Day 2 results 
Figure 12.2: Day 3 results 
Questions 

LAB 13: Epidemiology 
Results 
Questions 

APPENDIX 
Table A-1: Summary of Bacterial Strains 
Table A-2: Metabolic and Anatomic Differences between Cocci (Key) 
Table A-3: Metabolic Results on Selected Enterics Key 
Figure A-1: Bacillus subtilis (1000x stained with Gram stain) 
Figure A-2: Citrobacter freundii (1000x stained with Gram stain) 
Figure A-3: Enterobacter aerogenes (1000x stained with Gram stain) 
Figure A-4: Escherichia coli (1000x stained with Gram stain) 
Figure A-5: Klebsiella pneumonia (1000x stained with Gram stain) 
Figure A-6: Proteus vulgaris (1000x stained with Gram stain) 
Figure A-7: Salmonella typhimurium (1000x stained with Gram stain) 
Figure A-8: Sarcina lutea (1000x stained with Gram stain) 
Figure A-9: Serratia marcescens (1000x stained with Gram stain) 
Figure A-10: Shigella flexneri (1000x stained with Gram stain) 
Figure A-11: Staphylococcus aureus (1000x stained with Gram stain) 
Figure A-12: Staphylococcus epidermidis (1000x stained with Gram stain) 
Figure A-13: Streptococcus pyogenes (1000x stained with Gram stain)