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This publication is a microbiology laboratory manual designed for a one-semester, college undergraduate education. The manual is designed to be self-guided so that the students can learn and discover on their own time. All college majors requiring a microbiology laboratory course will benefit from this publication. This manual contains a series of experiments designed to build a student’s knowledge and mastery of microbiological laboratory techniques. It begins with basics of microscopy, aseptic technique, and culture handling, and then advances into diagnostic techniques applicable to many fields in microbiology. In addition, the manual has an unknown exercise that follows the student through the entire semester allowing for them to use various techniques and a dichotomous key to determine the identity of microorganisms.
LAB 1. INTRODUCTION TO MICROSCOPY
LAB 2. HANDWASHING
LAB 3. ASEPTIC TECHNIQUE AND PURE CULTURE TRANSFERS
LAB 4. SIMPLE STAIN
LAB 5. SURVEY OF EUKARYOTIC MICROORGANISMS
LAB 6. THE GRAM STAIN
LAB 7. SERIAL DILUTIONS (VIABLE PLATE COUNTS)
LAB 8. METABOLISM
LAB 9. METABOLISM 2 AND MOTILITY
LAB 10. SELECTIVE AND DIFFERENTIAL MEDIA
LAB 11. FOOD MICROBIOLOGY
LAB 12. WATER MICROBIOLOGY
LAB 13. ANTIMICROBIAL SUSCEPTIBILITY