Microbiology Lab Manual

Author(s): Janice Speshock

Edition: 5

Copyright: 2023

Pages: 86

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Ebook

$45.14

ISBN 9798765756423

Details Electronic Delivery EBOOK 180 days

This publication is a microbiology laboratory manual designed for a one-semester, college undergraduate education. The manual is designed to be self-guided so that the students can learn and discover on their own time. All college majors requiring a microbiology laboratory course will benefit from this publication. This manual contains a series of experiments designed to build a student’s knowledge and mastery of microbiological laboratory techniques. It begins with basics of microscopy, aseptic technique, and culture handling, and then advances into diagnostic techniques applicable to many fields in microbiology. In addition, the manual has an unknown exercise that follows the student through the entire semester allowing for them to use various techniques and a dichotomous key to determine the identity of microorganisms.

LAB 1. INTRODUCTION TO MICROSCOPY

LAB 2. HANDWASHING

LAB 3. ASEPTIC TECHNIQUE AND PURE CULTURE TRANSFERS

LAB 4. SIMPLE STAIN

LAB 5.  SURVEY OF EUKARYOTIC MICROORGANISMS

LAB 6. THE GRAM STAIN

LAB 7. SERIAL DILUTIONS (VIABLE PLATE COUNTS)

LAB 8.  METABOLISM

LAB 9.  METABOLISM 2 AND MOTILITY

LAB 10.  SELECTIVE AND DIFFERENTIAL MEDIA 

LAB 11.  FOOD MICROBIOLOGY

LAB 12.  WATER MICROBIOLOGY

LAB 13.  ANTIMICROBIAL SUSCEPTIBILITY 

APPENDICES

Janice Speshock

This publication is a microbiology laboratory manual designed for a one-semester, college undergraduate education. The manual is designed to be self-guided so that the students can learn and discover on their own time. All college majors requiring a microbiology laboratory course will benefit from this publication. This manual contains a series of experiments designed to build a student’s knowledge and mastery of microbiological laboratory techniques. It begins with basics of microscopy, aseptic technique, and culture handling, and then advances into diagnostic techniques applicable to many fields in microbiology. In addition, the manual has an unknown exercise that follows the student through the entire semester allowing for them to use various techniques and a dichotomous key to determine the identity of microorganisms.

LAB 1. INTRODUCTION TO MICROSCOPY

LAB 2. HANDWASHING

LAB 3. ASEPTIC TECHNIQUE AND PURE CULTURE TRANSFERS

LAB 4. SIMPLE STAIN

LAB 5.  SURVEY OF EUKARYOTIC MICROORGANISMS

LAB 6. THE GRAM STAIN

LAB 7. SERIAL DILUTIONS (VIABLE PLATE COUNTS)

LAB 8.  METABOLISM

LAB 9.  METABOLISM 2 AND MOTILITY

LAB 10.  SELECTIVE AND DIFFERENTIAL MEDIA 

LAB 11.  FOOD MICROBIOLOGY

LAB 12.  WATER MICROBIOLOGY

LAB 13.  ANTIMICROBIAL SUSCEPTIBILITY 

APPENDICES

Janice Speshock