The 2nd edition of the Microbiology Lab Manual by Mr. Roger Lighter and Dr. Joshua Burns is designed specifically for the General Microbiology course at the University of Arkansas-Fort Smith. Laboratory exercises have been designed and organized to provide students hands on experience with the tools and techniques of microbiological investigation. Biology majors will learn valuable lab skills that will help prepare them for upper level lab and research activities. At the same time, health science pre-professional students will gain the knowledge and skills essential for diagnosing disease in the clinic.
This course will cover a variety of topics including: methods of growing and culturing microorganisms, how to use and interpret selective and differential media, bacterial isolation procedures and aseptic techniques that are the basis of all universal precautions. In addition, students will participate in bacterial enumeration and ELISA labs, learn basic pipetting techniques and see the interdisciplinary nature of microbiology.
Laboratory Safety Guidelines
Lab Week #1 Microbiological Media and Culturing of Microorganisms
Lab Week #2 Isolation Streak, Microscope, and Simple Stain
Lab Week #3 Differential Stains: Gram Stain
Lab Week #4 Differential Stains: Endospore Stain and Environmental Effects on Growth
Lab Week #5 Capsule Stain (Negative Staining), Acid-Fast Stain, and Selective Media
Lab Week #6 Biochemical Tests: Differential Media and Pet ID
Lab Week #7 Serial Dilutions, and Plate Counting
Lab Week #8 Water Quality: Multiple Tube Fermentations and MPNs
Lab Week #9 Microbial Control: Disinfectants, Antibiotics, and UV Light
Lab Week #10 Immunology I: Blood Typing and White Blood Cell Counts
Lab Week #11 Immunology II: ELISA