Microbiology Lab Manual
Edition: 2
Copyright: 2015
Pages: 146
Edition: 2
Copyright: 2015
Pages: 146
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This laboratory manual provides hands-on experience with the fundamental techniques and practices used in microbiology. Students learn laboratory safety, microscopy, aseptic technique, microbial isolation and cultivation, staining methods, motility testing, culture methods, selective and differential media, biochemical identification, and antimicrobial susceptibility testing. Additional exercises examine environmental microbiology, hand hygiene, ultraviolet radiation, and the isolation and identification of clinically significant microorganisms.
General Rules for Microbiology Lab
Exercise 1: Use of the Brightfield Microscope
Exercise 2: Isolation of Microorganisms from the Environment
Exercise 3: The Negative Staining of Plaque
Exercise 4: Smear Preparation and Simple Staining
Exercise 5: Gram Staining
Exercise 6: Endospore (spore) Staining
Exercise 7: Acid-Fast Stain
Exercise 8: Motility Determination
Exercise 9: Pure Culture Methods
Exercise 10: Differential and Selective Media
Exercise 11: Biochemical Reactions
Exercise 12: Effectiveness of Hand-Washing
Exercise 13: Effects of Ultraviolet Radiation
Exercise 14: Disk Diffusion Method
Exercise 15: The Kirby-Bauer Test for Antimicrobic Sensitivity
Exercise 16: Isolation of Staphylococcus aureus
Exercise 17: Throat Cultures
Exercise 18: Review of Eukaryotic Pathogens
Exercise 19: Standard Plate Count
Lab Project One: An Investigation into Foodborne Illness Project
Lab Project Two: Identification of Prepared Unknowns Lab Project
Lab Project Three: Identification of Unknown from Broth Culture
Joseph Maciaszek is a Professor of Biological Sciences in the STEM Division at Springfield Technical Community College. He teaches biology and microbiology courses, helping students develop a foundation in the life sciences through lecture and laboratory instruction.
Monica Strzempko is an adjunct faculty member in the STEM Division at Springfield Technical Community College, where she teaches courses in the biological sciences. Her instruction emphasizes laboratory techniques, scientific inquiry, and foundational concepts that prepare students for further study and careers.