Microbiology Lab Manual

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Exercise 1. Lab Safety
Exercise 2. Handling and Examining Cultures
Exercise 3. Brightfield Microscopy
Exercise 4. Smear Preparation and Simple Staining Technique 
Exercise 5. Differential Staining: The Gram Stain 
Exercise 6. Special Differential Stains: Capsular Stain, Endospore Stain, and Acid Fast Stain
Exercise 7. Isolation of Bacteria 
Exercise 8. Selective and Differential Media for Isolation 
Exercise 9. Collection and Transport of Clinical Specimens 
Exercise 10. Biochemical Tests 
Exercise 11. Normal Flora of the Human Body 
Exercise 12. Normal Flora of the Skin 
Exercise 13. Normal Flora of the Nasal Passage 
Exercise 14. Bacterial Flora of the Respitory Tract 
Exercise 15. Normal Flora of the Throat 
Exercise 16. Physical Methods of Control 
Exercise 17. Chemical Methods of Control: Antiseptics and Disinfectants 
Exercise 18. Chemical Methods of Control: Antibiotic Susceptibility 
Exercise 19. Efficacy of Hand Washing

Dave S. Bachoon
Indiren Pillay

Exercise 1. Lab Safety
Exercise 2. Handling and Examining Cultures
Exercise 3. Brightfield Microscopy
Exercise 4. Smear Preparation and Simple Staining Technique 
Exercise 5. Differential Staining: The Gram Stain 
Exercise 6. Special Differential Stains: Capsular Stain, Endospore Stain, and Acid Fast Stain
Exercise 7. Isolation of Bacteria 
Exercise 8. Selective and Differential Media for Isolation 
Exercise 9. Collection and Transport of Clinical Specimens 
Exercise 10. Biochemical Tests 
Exercise 11. Normal Flora of the Human Body 
Exercise 12. Normal Flora of the Skin 
Exercise 13. Normal Flora of the Nasal Passage 
Exercise 14. Bacterial Flora of the Respitory Tract 
Exercise 15. Normal Flora of the Throat 
Exercise 16. Physical Methods of Control 
Exercise 17. Chemical Methods of Control: Antiseptics and Disinfectants 
Exercise 18. Chemical Methods of Control: Antibiotic Susceptibility 
Exercise 19. Efficacy of Hand Washing

Dave S. Bachoon
Indiren Pillay