Microbiology Laboratory: A Clinical Approach for Health Care Professionals
Edition: 3
Copyright: 2023
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New Fourth Edition Coming Soon!
We are constantly surrounded by ubiquitous, living organisms too small to be observed by the naked eye. Some of them are beneficial to us. They may serve us as part of our normal microflora competing with and inhibiting the growth of pathogenic microorganisms. Pathogenic and opportunistic microorganisms can cause sickness and diseases in humans, animals and plants. Microbiological techniques enable us to view and study these fascinating creatures.
Within Microbiology Laboratory: A Clinical Approach for Health Care Professionals by M. Nia Madison, PhD, students are exposed to an abundance of information critical for supplementing a knowledge base regarding microorganisms and the minute intricacies that enable them to survive and thrive in a myriad of environments. Throughout the text, students will:
- learn laboratory safety techniques which are of foremost importance to protecting themselves and others while handling potentially pathogenic microorganisms.
- become a master at inoculating differential and biochemical media using aseptic techniques to examine bacterial growth, respiration and metabolic attributes.
- master the art of various staining techniques, which, when paired with bright-field microscopy, can be used to examine bacterial morphology, arrangement and the presence of virulence factors including capsular material.
Microbiology Laboratory is ideal for both science majors and non-majors alike matriculating in either introductory or advanced microbiology laboratory courses. Its focus is on both general and applied microbiological techniques that will support the educational needs of students with an interest in science or in pursuing a career in a health care field such as medicine, dentistry and nursing.
Exercise 1 Introduction and Lab Safety
Exercise 2 Aseptic Technique
Exercise 3 Ubiquity of Bacteria
Exercise 4 Bacterial Characteristics
4.1 Bacterial Types and Morphology
4.2 Bacterial Cell Characteristics
4.3 Bacterial Diversity: Major Phyla and Key Genera
Exercise 5 Light Microscopy and Staining Techniques
5.1 Easy Use of the Brightfield (Light) Microscope
5.2 Microscope and Simple Stains
5.3 Differential Staining Techniques
5.4 Negative Stain with Nigrosin
5.5 Negative Stain with Congo Red
Exercise 6 Bacterial Growth
6.1 Bacterial Colony Morphology
6.2 Bacterial Motility
6.3 Cultural Characteristics of Bacteria
6.4 Pure Culture: Streak Plate Method
6.5 Antibiotic Susceptibility/Resistance
6.6 UVGI (2-Day Lab)
Exercise 7 Selective and Differential Growth Medium and Tests
7.1 Introduction to Identification of an Unknown Bacterial Species
7.2 Growth Patterns in Nutrient Broth and Agar Slants
7.3 Urease Test
7.4 Phenol Red Dextrose, Lactose, Sucrose and Gas Production Tests
7.5 Fluid Thioglycollate
7.6 Catalase Test
7.7 Oxidase Testy
7.8 Phenylalanine Deaminase Test
7.9 Hydrolytic Exoenzymes: Lipase and Casease
7.10 SIM Media: Sulfur Reduction, Indole Production, Motility
7.11 Mixed Acid Fermentation (Methyl Red Test) and Butanediol Fermentation (Voges-Proskauer Test
7.12 Citrate Utilization
7.13 Gelatin Hydrolysis
7.14 Starch Hydrolysis
Exercise 8 Bacteria of Clinical Importance
8.1 The Clinical Microbiologist: Identifying the Cause of an Infection
8.2 Dental Microbiology
8.3 Microbial Flora of the Throat, Nasopharynx, and Skin
8.4 Gastrointestinal Pathogens
8.5 Protozoa and Parasitic Helminths
8.6 Urine Culture
Exercise 9 Eukaryotic Microorganisms
9.1 Eukaryotes-Fungi
9.2 Fungi
9.3 Yeast and Mycelial Fungi
9.4 Eukaryotes-Parasites and Algae
9.5 Eukaryotic Parasites
9.6 Protista: Algae
9.7 Protista: Protozoa
Exercise 10 ABO/Rh Simulated Blood Typing
Dr. M. Nia Madison earned her B.S in biology with honors from East Texas Baptist University in 2000 and her Ph.D. in biomedical sciences, summa cum laude, from Meharry Medical College in Nashville, TN in 2008. She is an associate professor of microbiology lectures and labs at Miami Dade College, Homestead Campus in South Florida where she is also the Director of HIV research. Her research focus is on elucidating the identities of host-derived inhibitors and enhancers of HIV infectivity. She mentors undergraduate research interns in HIV research, laboratory techniques and research philosophy each semester. Also, an Earth conservationist, she utilizes her teaching platform to guide students on how to decrease their carbon footprint, decrease climate change and increase sustainability. She annually hosts a STEM workshop for 5-12th grade girls featuring research and lecture components by STEM-oriented, professional women as part of her goal to diversify STEM fields and inspire young girls to undertake STEM education and earn a terminal degree followed by a STEM oriented career. She was promoted in 2022 to the Simon Bolivar Endowed Teaching Chair, is the winner of the Faculty of the Year Award, the service learning rookie of the year award, the MDC President’s Excellence award, the 2021 and 2022 MDC President’s Innovation Award, The 2021 and 2022 AAUW STEM Ambassadorship, the 2020-2022 AAAS If/Then STEM Ambassadorship, the 2021 Bob Graham Distinguished Faculty Award from the Florida College System Activities Association, and the 2019 East Texas Baptist University, Young Alumnus Award.
"Live your life with purpose, vision, impact, service to others and service to our planet; it will be a life well-lived with an everlasting legacy". -M. Nia Madison
New Fourth Edition Coming Soon!
We are constantly surrounded by ubiquitous, living organisms too small to be observed by the naked eye. Some of them are beneficial to us. They may serve us as part of our normal microflora competing with and inhibiting the growth of pathogenic microorganisms. Pathogenic and opportunistic microorganisms can cause sickness and diseases in humans, animals and plants. Microbiological techniques enable us to view and study these fascinating creatures.
Within Microbiology Laboratory: A Clinical Approach for Health Care Professionals by M. Nia Madison, PhD, students are exposed to an abundance of information critical for supplementing a knowledge base regarding microorganisms and the minute intricacies that enable them to survive and thrive in a myriad of environments. Throughout the text, students will:
- learn laboratory safety techniques which are of foremost importance to protecting themselves and others while handling potentially pathogenic microorganisms.
- become a master at inoculating differential and biochemical media using aseptic techniques to examine bacterial growth, respiration and metabolic attributes.
- master the art of various staining techniques, which, when paired with bright-field microscopy, can be used to examine bacterial morphology, arrangement and the presence of virulence factors including capsular material.
Microbiology Laboratory is ideal for both science majors and non-majors alike matriculating in either introductory or advanced microbiology laboratory courses. Its focus is on both general and applied microbiological techniques that will support the educational needs of students with an interest in science or in pursuing a career in a health care field such as medicine, dentistry and nursing.
Exercise 1 Introduction and Lab Safety
Exercise 2 Aseptic Technique
Exercise 3 Ubiquity of Bacteria
Exercise 4 Bacterial Characteristics
4.1 Bacterial Types and Morphology
4.2 Bacterial Cell Characteristics
4.3 Bacterial Diversity: Major Phyla and Key Genera
Exercise 5 Light Microscopy and Staining Techniques
5.1 Easy Use of the Brightfield (Light) Microscope
5.2 Microscope and Simple Stains
5.3 Differential Staining Techniques
5.4 Negative Stain with Nigrosin
5.5 Negative Stain with Congo Red
Exercise 6 Bacterial Growth
6.1 Bacterial Colony Morphology
6.2 Bacterial Motility
6.3 Cultural Characteristics of Bacteria
6.4 Pure Culture: Streak Plate Method
6.5 Antibiotic Susceptibility/Resistance
6.6 UVGI (2-Day Lab)
Exercise 7 Selective and Differential Growth Medium and Tests
7.1 Introduction to Identification of an Unknown Bacterial Species
7.2 Growth Patterns in Nutrient Broth and Agar Slants
7.3 Urease Test
7.4 Phenol Red Dextrose, Lactose, Sucrose and Gas Production Tests
7.5 Fluid Thioglycollate
7.6 Catalase Test
7.7 Oxidase Testy
7.8 Phenylalanine Deaminase Test
7.9 Hydrolytic Exoenzymes: Lipase and Casease
7.10 SIM Media: Sulfur Reduction, Indole Production, Motility
7.11 Mixed Acid Fermentation (Methyl Red Test) and Butanediol Fermentation (Voges-Proskauer Test
7.12 Citrate Utilization
7.13 Gelatin Hydrolysis
7.14 Starch Hydrolysis
Exercise 8 Bacteria of Clinical Importance
8.1 The Clinical Microbiologist: Identifying the Cause of an Infection
8.2 Dental Microbiology
8.3 Microbial Flora of the Throat, Nasopharynx, and Skin
8.4 Gastrointestinal Pathogens
8.5 Protozoa and Parasitic Helminths
8.6 Urine Culture
Exercise 9 Eukaryotic Microorganisms
9.1 Eukaryotes-Fungi
9.2 Fungi
9.3 Yeast and Mycelial Fungi
9.4 Eukaryotes-Parasites and Algae
9.5 Eukaryotic Parasites
9.6 Protista: Algae
9.7 Protista: Protozoa
Exercise 10 ABO/Rh Simulated Blood Typing
Dr. M. Nia Madison earned her B.S in biology with honors from East Texas Baptist University in 2000 and her Ph.D. in biomedical sciences, summa cum laude, from Meharry Medical College in Nashville, TN in 2008. She is an associate professor of microbiology lectures and labs at Miami Dade College, Homestead Campus in South Florida where she is also the Director of HIV research. Her research focus is on elucidating the identities of host-derived inhibitors and enhancers of HIV infectivity. She mentors undergraduate research interns in HIV research, laboratory techniques and research philosophy each semester. Also, an Earth conservationist, she utilizes her teaching platform to guide students on how to decrease their carbon footprint, decrease climate change and increase sustainability. She annually hosts a STEM workshop for 5-12th grade girls featuring research and lecture components by STEM-oriented, professional women as part of her goal to diversify STEM fields and inspire young girls to undertake STEM education and earn a terminal degree followed by a STEM oriented career. She was promoted in 2022 to the Simon Bolivar Endowed Teaching Chair, is the winner of the Faculty of the Year Award, the service learning rookie of the year award, the MDC President’s Excellence award, the 2021 and 2022 MDC President’s Innovation Award, The 2021 and 2022 AAUW STEM Ambassadorship, the 2020-2022 AAAS If/Then STEM Ambassadorship, the 2021 Bob Graham Distinguished Faculty Award from the Florida College System Activities Association, and the 2019 East Texas Baptist University, Young Alumnus Award.
"Live your life with purpose, vision, impact, service to others and service to our planet; it will be a life well-lived with an everlasting legacy". -M. Nia Madison