Microbiology Laboratory Manual

PART I

LABORATORY ORIENTATION AND BASIC TECHNIQUES

1. General Rules of the Lab

2. The Compound Light Microscope

3. Microscopic Measurement

4. Cultural Characteristics of Bacteria

5. Preparation and Care of Stock Cultures, and Aseptic Technique

PART II

STAINING AND SLIDE TECHNIQUES

6. The Negative Stain

7. Preparing a Bacterial Smear

8. The Simple Stain

9. The Gram Stain

10. Structural Stains (Endospore and Capsule)

11. Acid-Fast Stain

12. Motility Determination

PART III

CULTURE TECHNIQUES

13. Working With Media

14. Isolating Pure Cultures

15. Bacterial Population Counts

16. Cultivation of Anaerobic Bacteria

PART IV

MICROORGANISMS AND DISEASE

17. Selective and Differential Media

17. Alternate Method for Differential and Selective Media

18. The Staphylococci

19. The Streptococci

20. Urinary Pathogens

21. Dental Caries Susceptibility

21. Alternate Method for Dental Caries Susceptibility

22. Bacteria of the Skin

PART V

MICROORGANISMS IN THE ENVIRONMENT

23. Ubiquity of Microorganisms

24. Bacteria in Water

25. Bacteria in Milk

26. Bacteria in Food

27. Bacteria in Soil

PART VI

IDENTIFICATION OF UNKNOWN BACTERIA

28. Morphological Study of Unknown

29. Physiological Characteristics: Biooxidations

30. Physiological Characteristics: Hydrolysis

31. Physiological Characteristics: Miscellaneous Tests

PART VII

CONTROL OF MICROBIAL GROWTH

32. Effects of Temperature on Growth

33. Effects of Ultraviolet Light

34. Antimicrobics

35. Effects of Antiseptics and Disinfectants

36. Kingdom Fungi

37. Kingdom Protista—Protozoa and Slime Molds

38. Parasitic Worm Infections of Humans

APPENDICES

A. Laboratory Orientation

B. Spectrophotometry

C. Further Studies

I. Making Wine

II. Preparation of Sauerkraut

III. Cheese Microbiology

IV. Effectiveness of a Sewage Treatment Plant

V. Petroleum Use by Bacteria

VI. Contamination of a Drinking Glass

D. Additional Information on Parasitic Infections of Humans

E. List of Media