Microbiology Laboratory Manual
Edition: 2
Copyright: 2019
Pages: 212
Edition: 2
Copyright: 2019
Pages: 212
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The importance of microbiology to biology majors becomes more, and more evident as new research points to critical linkages between humans and microbial communities found in our bodies. As biology students become more aware of the microbial communities in our bodies, or the importance they play in the health of our environment it is critical that they have a good appreciation of the laboratory side of the field of microbiology to understand the methods we use to learn about microorganisms. Microbiology Laboratory for Biology Majors will allow a biology major to start the process of working with bacteria in a laboratory setting safely. By conducting exercises that include the isolation of pure bacterial cultures, staining bacteria, and identification of unknown bacterial cultures students will learn procedures that are utilized by microbiologists the world over on a daily basis. Through exercises that focus on environmental microbiology students may also learn to appreciate how microbial ecologists have helped develop an understanding of the roles microbes play in natural ecosystems. Hopefully, as a student completes a microbiology lab course utilizing this manual, their curiosity about microorganisms related to many human activities will stimulate their desire to learn more about the little, invisible microorganisms that surround us, both inside and out.
General Microbiology Exercises
Laboratory Safety
Laboratory Safety
Exercise 1: Microscopy
Exercise 2: Preparation of Bacterial Culture Media
Exercise 3: Aseptic Technique
Exercise 4: Pure Cultures
Exercise 5: Staining Bacteria
Exercise 6: Bacterial Enumeration
Exercise 7: Bacterial Growth
Exercise 8: Selective and Differential Media
Exercise 9: Media Demonstrating Unique Enzymes
Exercise 10: Media Demonstrating Carbohydrate Metabolism
Exercise 11: Identification of Unknown Bacterial Cultures
Exercise 12: Environmental Influences on Bacterial Growth
Exercise 13: Bacterial Death
Exercise 14: Isolation of Microorganisms from the Human Body
Environmental Microbiology Exercises
Rationale For Environmental microbiology laboratory exercises
Basic Laboratory Radiation Safety
Basic Laboratory Radiation Safety
Laboratory Report Instructions
Citations in Your Report
Microbial Ecology Lab Exercises
Exercise 15: Assessment of Bacteria Present in an Aquatic Habitat
Exercise 16: Assessment of Bacteria Present in a Soil or Sediment Habitat
Exercise 17: Glucose Mineralization – An Indication of Heterotrophic Potential
Bioremediation Lab Exercises
Exercise 18: Microbial Dye Degradation
Exercise 19: Herbicide Mineralization
Exercise 20: Degradation of Waste Oil by Soil or Sediment Microorganisms
Appendix A: Laboratory Culture List
Appendix B: Enrichment Cultures & Basal Salts Medium
Appendix C: Hexane Extractions for Aqueous Samples
Dr. Spratt’s training and professional experience fall mostly in the field of Microbial Ecology. He has studied transformations of various elements (e.g., C, N, S, Fe, Mn) by microorganisms in forest soils and wetland sediments, ranging from tropical mangrove swamps to peat bogs. Recently, he shifted the focus of his research to studies of pathogenic bacteria found in clinical environments. His research interest in these clinical settings is microbial ecology of the human, built, rather than natural environment. He still conducts research in natural settings, but through partnerships with other researchers at the University of Tennessee at Chattanooga (UTC) and with local physicians Dr. Spratt helped establish a UTC research group called the Clinical Infectious Disease Control (CIDC) group in 2015. The CIDC currently has grant support for multiple research projects. Ultimately, the CIDC hopes to focus study on potential links between pathogens present in the clinical environment with healthcare associated infections (HAIs), providing clinic managers with options to control those pathogens and reduce HAIs.
He has published his biogeochemical research in national and international journals (e.g., Applied and Environmental Microbiology; Estuarine, Coastal, and Shelf Science; Limnology and Oceanography). The CIDC has published five articles in nationally recognized journals, and currently have three manuscripts in preparation. The CIDC has also written three articles for blogs associated with professional societies.
Dr. Spratt has taught university courses ranging from freshmen introductory biology, to graduate courses in microbial physiology. He routinely teaches pre-med students their basic microbiology course, as well as their introduction to immunology. He has taught two graduate courses, microbial ecology, and bioremediation for over 20 years. In 2022 he published a textbook based on his bioremediation course (Bioremediation: Cleaning toxic wastes with a little help from nature. KendallHunt, ISBN: 979-8-7657-0772-2). He has mentored 20 graduate students through UTC's MS in Environmental Science since the program's inception in 1997.
Specialties: Prokaryotic biology, microbial ecology, clinical microbiology, undergraduate education, environmental education
New Edition Now Available!
The importance of microbiology to biology majors becomes more, and more evident as new research points to critical linkages between humans and microbial communities found in our bodies. As biology students become more aware of the microbial communities in our bodies, or the importance they play in the health of our environment it is critical that they have a good appreciation of the laboratory side of the field of microbiology to understand the methods we use to learn about microorganisms. Microbiology Laboratory for Biology Majors will allow a biology major to start the process of working with bacteria in a laboratory setting safely. By conducting exercises that include the isolation of pure bacterial cultures, staining bacteria, and identification of unknown bacterial cultures students will learn procedures that are utilized by microbiologists the world over on a daily basis. Through exercises that focus on environmental microbiology students may also learn to appreciate how microbial ecologists have helped develop an understanding of the roles microbes play in natural ecosystems. Hopefully, as a student completes a microbiology lab course utilizing this manual, their curiosity about microorganisms related to many human activities will stimulate their desire to learn more about the little, invisible microorganisms that surround us, both inside and out.
General Microbiology Exercises
Laboratory Safety
Laboratory Safety
Exercise 1: Microscopy
Exercise 2: Preparation of Bacterial Culture Media
Exercise 3: Aseptic Technique
Exercise 4: Pure Cultures
Exercise 5: Staining Bacteria
Exercise 6: Bacterial Enumeration
Exercise 7: Bacterial Growth
Exercise 8: Selective and Differential Media
Exercise 9: Media Demonstrating Unique Enzymes
Exercise 10: Media Demonstrating Carbohydrate Metabolism
Exercise 11: Identification of Unknown Bacterial Cultures
Exercise 12: Environmental Influences on Bacterial Growth
Exercise 13: Bacterial Death
Exercise 14: Isolation of Microorganisms from the Human Body
Environmental Microbiology Exercises
Rationale For Environmental microbiology laboratory exercises
Basic Laboratory Radiation Safety
Basic Laboratory Radiation Safety
Laboratory Report Instructions
Citations in Your Report
Microbial Ecology Lab Exercises
Exercise 15: Assessment of Bacteria Present in an Aquatic Habitat
Exercise 16: Assessment of Bacteria Present in a Soil or Sediment Habitat
Exercise 17: Glucose Mineralization – An Indication of Heterotrophic Potential
Bioremediation Lab Exercises
Exercise 18: Microbial Dye Degradation
Exercise 19: Herbicide Mineralization
Exercise 20: Degradation of Waste Oil by Soil or Sediment Microorganisms
Appendix A: Laboratory Culture List
Appendix B: Enrichment Cultures & Basal Salts Medium
Appendix C: Hexane Extractions for Aqueous Samples
Dr. Spratt’s training and professional experience fall mostly in the field of Microbial Ecology. He has studied transformations of various elements (e.g., C, N, S, Fe, Mn) by microorganisms in forest soils and wetland sediments, ranging from tropical mangrove swamps to peat bogs. Recently, he shifted the focus of his research to studies of pathogenic bacteria found in clinical environments. His research interest in these clinical settings is microbial ecology of the human, built, rather than natural environment. He still conducts research in natural settings, but through partnerships with other researchers at the University of Tennessee at Chattanooga (UTC) and with local physicians Dr. Spratt helped establish a UTC research group called the Clinical Infectious Disease Control (CIDC) group in 2015. The CIDC currently has grant support for multiple research projects. Ultimately, the CIDC hopes to focus study on potential links between pathogens present in the clinical environment with healthcare associated infections (HAIs), providing clinic managers with options to control those pathogens and reduce HAIs.
He has published his biogeochemical research in national and international journals (e.g., Applied and Environmental Microbiology; Estuarine, Coastal, and Shelf Science; Limnology and Oceanography). The CIDC has published five articles in nationally recognized journals, and currently have three manuscripts in preparation. The CIDC has also written three articles for blogs associated with professional societies.
Dr. Spratt has taught university courses ranging from freshmen introductory biology, to graduate courses in microbial physiology. He routinely teaches pre-med students their basic microbiology course, as well as their introduction to immunology. He has taught two graduate courses, microbial ecology, and bioremediation for over 20 years. In 2022 he published a textbook based on his bioremediation course (Bioremediation: Cleaning toxic wastes with a little help from nature. KendallHunt, ISBN: 979-8-7657-0772-2). He has mentored 20 graduate students through UTC's MS in Environmental Science since the program's inception in 1997.
Specialties: Prokaryotic biology, microbial ecology, clinical microbiology, undergraduate education, environmental education