Microbiology Laboratory Manual

PART I LABORATORY ORIENTATION AND BASIC TECHNIQUES
1. General Rules of the Lab
2. The Compound Light Microscope
3. Microscopic Measurement
4. Cultural Characteristics of Bacteria
5. Preparation and Care of Stock Cultures, and Aseptic Technique

PART II STAINING AND SLIDE TECHNIQUES
6. The Negative Stain
7. Preparing a Bacterial Smear
8. The Simple Stain
9. The Gram Stain
10. Structural Stains (Endospore and Capsule)
11. Acid-Fast Stain
12. Motility Determination

PART III CULTURE TECHNIQUES
13. Working With Media
14. Isolating Pure Cultures
15. Bacterial Population Counts
16. Cultivation of Anaerobic Bacteria

PART IV MICROORGANISMS AND DISEASE
17. Selective and Differential Media
17. Alternate Method for Differential and Selective Media
18. The Staphylococci
19. The Streptococci
20. Urinary Pathogens
21. Dental Caries Susceptibility
21. Alternate Method for Dental Caries Susceptibility
22. Bacteria of the Skin

PART V MICROORGANISMS IN THE ENVIRONMENT
23. Ubiquity of Microorganisms
24. Bacteria in Water
25. Bacteria in Milk
26. Bacteria in Food
27. Bacteria in Soil

PART VI IDENTIFICATION OF UNKNOWN BACTERIA
28. Morphological Study of Unknown
29. Physiological Characteristics: Biooxidations
30. Physiological Characteristics: Hydrolysis  
31. Physiological Characteristics: Miscellaneous Tests

PART VII CONTROL OF MICROBIAL GROWTH
32. Effects of Temperature on Growth
33. Effects of Ultraviolet Light
34. Antimicrobics
35. Effects of Antiseptics and Disinfectants
36. Kingdom Fungi
37. Kingdom Protista—Protozoa and Slime Molds
38. Parasitic Worm Infections of Humans

APPENDICES
A. Laboratory Orientation
B. Spectrophotometry

C. Further Studies
I. Making Wine
II. Preparation of Sauerkraut
III. Cheese Microbiology
IV. Effectiveness of a Sewage Treatment Plant
V. Petroleum Use by Bacteria
VI. Contamination of a Drinking Glass 

D. Additional Information on Parasitic Infections of Humans
E. List of Media