Microbiology Laboratory Manual

PART I 

LABORATORY ORIENTATION AND BASIC TECHNIQUES 1

1. General Rules of the Lab 3 

2. The Compound Light Microscope 9 

3. Cultural Characteristics of Bacteria 21 

4. Preparation and Care of Stock Cultures, and Aseptic Technique 27
PART II 

STAINING AND SLIDE TECHNIQUES 37
5. Preparing a Bacterial Smear 39 

6. The Simple Stain 45

7. The Gram Stain 49 

8. Structural Stains (Endospore and Capsule) 57 

9. Acid-Fast Stain 71
10. Motility Determination 75
PART III 

CULTURE TECHNIQUES 79
11. Isolating Pure Cultures 81 

PART IV 

MICROORGANISMS AND DISEASE 91
12. Selective and Differential Media 93 

12. Alternate Method for Differential and Selective Media 97 

13. The Staphylococci 101

14. The Streptococci 109