Microbiology: Theory and Application

Chapter 1 Introduction and History of Microbiology 
Lab 1.1 Introduction to the Microbiology Laboratory 
Lab 1.2 Environmental Sampling and Normal Microbiota 
Lab 1.3 Microscopy 

Chapter 2 Classification of Microbes and Survey of the Microbial World 

Chapter 3 Bacterial Cell Structure and Function 
Lab 3.1 Smear Preparation, Gram Staining, and Laboratory Diagnosis 
Lab 3.2 Endospores and Hypothesis Testing 
Lab 3.3 Acid-Fast Stain 
Lab 3.4 Endospore Stain 
Lab 3.5 Capsule and Flagella Stains 
Lab 3.6 Biofilm and Microbial Communities 

Chapter 4 Media and Lab Techniques 
Lab 4.1 Streak Plate Technique, Pure Culture Techniques, Colony Characteristics and Selective and Differential Media 

Chapter 5 Microbial Growth and Nutrition 
Lab 5.1 Measuring Microbial Growth, Nutritional Requirements for Growth, and Optimal Conditions for Growth 

Chapter 6 The Ecological Importance of Microbes 

Chapter 7 Control of Microbial Growth 
Lab 7.1 Control Methods: Effectiveness of Chemical and Physical Controls 
Lab 7.2 Water Quality Analysis 
Lab 7.3 Food Quality Analysis and Bacterial Population Counts in Food 

Chapter 8 Microbial Metabolism 
Lab 8.1 Metabolism: Enzyme Action, Carbohydrate Catabolism, and Introduction to Biochemical Testing 

Chapter 9 Genetics 
Lab 9.1 Genetics: Gene Expression and Phenotypic Variation 
Lab 9.2 Genetics: Transformation 

Chapter 10 Biotechnology 
Lab 10.1 Genetics: DNA Isolation and DNA Technology 
Lab 10.2 Agarose Gel Electrophoresis of DNA

Chapter 11 Antimicrobial Drugs 
Lab 11.1 Control Methods: Antibiotic Testing 

Chapter 12 Acellular Agents: Viruses, Viroids, and Prions 
Lab 12.1 Bacteriophages 

Chapter 13 Disease and Microbial Mechanisms of Pathogenesis
Lab 13.1 Medical Microbiology: Urinalysis and Sexually Transmitted Diseases 
Lab 13.2 Medical Microbiology: Throat Culture Activity 

Chapter 14 Epidemiology
Lab 14.1 Disease Transmission and Epidemiology 

Chapter 15 The Immune System 
Lab 15.1 Medical Microbiology: Innate Immunity: The Role of Normal Microbiota and Antimicrobial Activity of the Skin 
Lab 15.2 Medical Microbiology: Blood, Immunology, and the Differential White Blood Cell Count 

Appendix 1 The Unknown Project 
Appendix 2 Identification Chart 
Appendix 3 Microbes Used in the Lab

Index