Microorganisms: A Laboratory Manual
Edition: 3
Copyright: 2018
Edition: 3
Copyright: 2018
Edition: 3
Copyright: 2018
Pages: 248
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This edition of Microorganisms: A Laboratory Manual has been enhanced through the addition of clear learning objectives for each laboratory experience. All of the experiments have been student-tested and modified whenever necessary. The last few weeks of the course are devoted to the identification of unknowns, where the student gets an opportunity to use what was learned during the semester. The goals of the course are to prepare students for upper division microbiology and molecular biology courses both here and elsewhere, for careers in medical and cytotechnology, nursing, and clinical microbiology, for curricula in medical, dental and veterinary school and to provide broad, general microbiology training for those students for whom this is their only microbiology course.
Preface
Laboratory Safety
SECTION ONE— INTRODUCTORY TECHNIQUES
Laboratory 1—Metric System and Microscopes
Laboratory 2—Culture Media
Laboratory 3—Inoculation of Media; Aseptic Technique
Laboratory 4—Bacteria: Nomenclature, Colony Morphology
SECTION TWO—STAINING TECHNIQUES
Laboratory 5—Negative Stain
Laboratory 6—Smear Preparation
Laboratory 7—Simple Stain
Laboratory 8—Gram Stain
Laboratory 9—Endospore Stain
Laboratory 10—Acid-Fast Stain
SECTION THREE—BACTERIAL GROWTH AND CONTROL
Laboratory 11—Bacterial Population Count
Laboratory 12—Temperature
Laboratory 13—Ultraviolet Radiation
Laboratory 14—Oxygen Tolerance
Laboratory 15—pH
Laboratory 16—Osmolarity
SECTION FOUR—ANTIMICROBIALS AND EPIDEMIOLOGY
Laboratory 17—Antiseptics and Disinfectants
Laboratory 18—Antibiotic Susceptibility
Laboratory 19—Epidemiology
SECTION FIVE—SURVEY OF MICROORGANISMS
Laboratory 20—Fungi and Lichens
Laboratory 21—Cyanobacteria, Algae, and Protozoa
SECTION SIX—ENVIRONMENTAL MICROBIOLOGY
Laboratory 22—Water Analysis Membrane Filtration, Colisure Test, MPN
Laboratory 23—Microbial Interrelationships
SECTION SEVEN—MICROBIAL GENETICS
Laboratory 24—Transformation
Laboratory 25—Genetic