This edition of Microorganisms: A Laboratory Manual has been enhanced through the addition of clear learning objectives for each laboratory experience. All of the experiments have been student-tested and modified whenever necessary. The last few weeks of the course are devoted to the identification of unknowns, where the student gets an opportunity to use what was learned during the semester. The goals of the course are to prepare students for upper division microbiology and molecular biology courses both here and elsewhere, for careers in medical and cytotechnology, nursing, and clinical microbiology, for curricula in medical, dental and veterinary school and to provide broad, general microbiology training for those students for whom this is their only microbiology course.
Laboratory 1—Microscopes
Laboratory 2—Culture Media
Laboratory 3—Inoculation of Media; Aseptic Technique
Laboratory 4—Bacteria: Nomenclature, Colony Morphology
Laboratory 5—Negative Stain
Laboratory 6-8—Bacterial Smear, Simple Stain and Gram Stain
Laboratory 9-10—Endospore Stain and Acid-Fast Stain
Laboratory 11—Bacterial Population Count
Laboratory 12—Temperature
Laboratory 13—Ultraviolet Radiation
Laboratory 14—Oxygen
Laboratory 15—pH
Laboratory 16—Osmolarity
Laboratory 17—Antiseptics and Disinfectants
Laboratory 18—Antibiotic Susceptibility
Laboratory 19—Epidemiology
Laboratory 20—Fungi, Lichens and Filamentous Bacteria
Laboratory 21—Cyanobacteria, Algae and Protozoa
Laboratory 22—Membrane Filtration and Colisure Test
Laboratory 23—Microbial Interrelationships
Laboratory 24—Transformation
Laboratory 25—Conjugation
Laboratory 26—Bacteriophage
Laboratory 27-30—Biochemical Tests
Laboratory 31—Unknown Identification