This laboratory manual provides students with hands-on experience in fundamental techniques of molecular and cell biology, reinforcing core concepts through practical application. Beginning with essential skills such as solution preparation and pH monitoring, the manual progresses through key experimental methods including spectrophotometry, PCR-based GMO detection, protein isolation and purification, subcellular fractionation, and enzyme kinetics. Each chapter is designed to build technical proficiency and critical thinking, culminating in guidance on scientific report writing. Emphasizing both molecular and cellular approaches, this manual prepares students for advanced study and research in the biological sciences.
CHAPTER 1 Solution-Making and pH Monitoring
CHAPTER 2 Spectrophotometry
CHAPTER 3 Detection of Genetically Modified Foods (GMOs) Using Polymerase Chain Reaction (PCR)
CHAPTER 4 Isolation of Proteins From Spinach Leaves
Salt Precipitations
Ion Exchange Chromatography
PAGE and Protein Transfer
CHAPTER 5 Subcellular Fractionation
CHAPTER 6 Enzyme Kinetics
CHAPTER 7 Guidelines for Writing a Report