Biochemistry Lab Manual
Edition: 5
Copyright: 2021
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Introduction 1
Chapter 1 - Photometric Methods for Protein Determination 15
Chapter 2 - Buffers and Titrations 40
Chapter 3 - Purification of an Enzyme 67
Chapter 4 - Functional Characteristics of Lactate Dehydrogenase - Kinetics - Km and Vmax and the Effect of Inhibitors 108
Chapter 5 - Structural Characterization of Lactate Dehydrogenase 126
Chapter 6 - Isolation of Plasmid DNA and Restriction Mapping 182
Chapter 7 - Carbohydrates: Quantitative Determination of Sugar in Blood 210
Chapter 8 - In Vitro Transcription and Translation 229
Chapter 9 - Protein Modification 268
Chapter 10 - Lipids and Membranes 324
Chapter 11 - Molecular Modeling 373