The laboratories included in Cell Biology: A Research-Based Approach are primarily designed to introduce students to a number of standard, yet powerful, experimental techniques used in the modern-day cell biology laboratory. Notably, however, they are also organized as a set of experiments designed to shed light on a completely uncharacterized cellular protein denominated FAM171B.
Therefore, these labs allow students to perform "real research" trying to decipher the normal cellular function of this novel protein. FAM171B was originally identified via human genome sequencing studies; however, it has yet to be functionally characterized. Indeed, very little has been published on this protein to date. Thus, the experiments in this set of labs should be considered “primary research” studies whose intent is to help elucidate FAM171B’s protein structure and particularly its intracellular localization; each of which might provide clues to its molecular function within cells.
Introduction to Cell Biology Lab
Lab 1: Laboratory Safety
Lab 2: Bioinformatics
Lab 3: Subcellular Fractionation
Lab 4: Protein Assay
Lab 5: SDS/PAGE
Lab 6: Western/Immunoblot (Part 1)
Lab 7: Western/Immunoblot (Part 2)
Lab 8: Cell Culture
Lab 9: Immunofluorescence (Part 1)
Lab 10: Immunofluorescence (Part 2)
Appendix: Reagent and Supply List
Geoffrey M.
Goellner
Dr. Geoffrey Goellner is a Professor in the Department of Biology at Minnesota State University, Mankato, where he teaches courses in cell biology, neurobiology, and human physiology. He also serves as a faculty advisor for pre-medical and pre-dental students, providing academic and career guidance for students pursuing healthcare professions.
He earned his Ph.D. from the Mayo Foundation in Rochester, Minnesota. His research focuses on polyglutamine-containing proteins and their relationship to neurodegenerative diseases such as Huntington’s disease. In his laboratory, he works with graduate and undergraduate students using cell and molecular biology techniques to investigate protein function and mechanisms involved in neurodegeneration.
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