Small World Initiative: Research Protocols and Guide PACKAGE

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Edition: 4

Copyright: 2016

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$24.00

ISBN 9798385157860

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"Two-book set/package contains Research Protocols print (Paperback: 66 pages with digital e-Book access), and Research Guide print (Paperback: 116 pages, with digital e-Book access).

 

This two-book set is part of the official materials used in the Small World Initiative¨ (SWI). Initiated at Yale University in 2012, SWI is an innovative program that inspires and retains students in the sciences by engaging them in the hunt to find new drugs to treat infectious diseases known as superbugs.

 

""Infectious disease threats, like the coronavirus and superbugs, undermine modern medicine,"" according to SWI's President, Erika Kurt. ""Leveraging the collective efforts of thousands of students and educators around the world gives us a real chance to tackle these challenges head on and provides an incredible STEM education in the process."" As COVID-19 has demonstrated, a lack of preparedness can take a huge human, financial, and educational toll, making the Small World Initiative's work critical to our fight against the next pandemic.

 

Differentiating itself from traditional science education programs, SWI's program provides original research opportunities rather than relying on cookbook experiments with predetermined results. Students are introduced to discovery-based research through the core research project of antibiotic discovery from soil microbes.

 

Through a series of experiments, students collect soil samples, isolate diverse bacteria, test their bacteria against clinically-relevant microorganisms, and characterize those showing inhibitory activity.

 

While most antibiotics come from soil and it remains a great place to look for new ones, finding new candidates requires screening thousands of samples.

 

SWI's approach provides the requisite platform to crowdsource antibiotic discovery by tapping into the power of thousands of student researchers concurrently addressing a global challenge.

 

This unique approach harnesses the power of active learning to achieve both educational and scientific goals. Since its inception, SWI has impacted hundreds of schools across the United States and around the world and helps them transform science education and promote antibiotic discovery through the curiosity and inventiveness of young scientists.

 

""SWI allows students to not just dream about using science to save lives, but to actually dig in and help save the world, starting at school,"" says Kurt. ""The program is an example of how groundbreaking research can start in the classroom."""

Volume 1

Acknowledgements

Small World Initiative Overview

Introduction: The Antibiotic Crisis

Section 1: Living on a Bacterial Planet
          Experiment 1: Devise a method to transfer microbes from a soil sample to a medium in the lab

Section 2: More than just “Dirt”
          Experiment 2: Find a local* soil environment you wish to sample

Section 3: Redefining “Growth” and “Culture”
          Experiment 3: Find a method to isolate single colonies of bacteria from your soil sample

Section 4: Bacteria Are What They Eat, Too
          Experiment 4: Choose your own media na dculture conditions

Section 5: Solid Versus Liquid Cultures
          Experiment 5: Isolate unique colonies to test for antibiotic production

Section 6: Meet the ESKAPE Pathogens
          Experiment 6: Understand the significance of the ESKAPE pathogens and using safe relatives in the lab

Section 7: Antibiotic Discovery, Structure, & Targets
          Experiment 7: Design a method to screen for antibiotic producers

Section 8: Getting to Know Your Isolates
          Experiment 8: Conduct initial identification of your antibiotic-producing isolate

Section 9: It All Comes Down to Chemistry
          Experiment 9: Test an organic extract of your isolate for antibiotic activity

Section 10: Resisting Antibiotics
          Experiment 10: Test your isolate’s resistance to common antibiotics

Section 11: “Classic” versus “Modern”
          Experiment 11: Conduct biochemical characterization of your isolates

Section 12: Bacteria in Context
          Experiment 12: Assess your isolate’s activity against eukaryotes, potential use as biological control, and ecological relationships with other organisms

Future Directions

Concluding Remarks

 

Volume 2

Acknowledgements

Lab Safety and Best Practices

Agarose Gel Electrophoresis

Aligning and Combining 16S rRNA Gene Sequences

Analyzing Organic Extracts for Antibiotic Production

Analyzing Sequences with BLAST Search 

Antibiotic Resistance Test

Catalase Test

Colony Morphology

Colony PCR

Fermentation

Gram Stain

MacConkey Agar Test

Making Glycerol Stocks

Methanol Extraction 

Obtaining Soil Sample 

Picking and Patching Colonies

Plating Soil Sample

Screen for Isolate Antibiotic Production #1 – Patch/Patch

Screen for Isolate Antibiotic Production #2 – Spread/Patch

Screen for Isolate Antibiotic Production #3 – Top Agar

Serial Dilutions

Silica Column Chromatography Protocol

Spread Plate 

Streak Plate

Sulfide and Indole Production and Motility

Thin Layer Chromatography (TLC) 

Typical Media Menu

"Two-book set/package contains Research Protocols print (Paperback: 66 pages with digital e-Book access), and Research Guide print (Paperback: 116 pages, with digital e-Book access).

 

This two-book set is part of the official materials used in the Small World Initiative¨ (SWI). Initiated at Yale University in 2012, SWI is an innovative program that inspires and retains students in the sciences by engaging them in the hunt to find new drugs to treat infectious diseases known as superbugs.

 

""Infectious disease threats, like the coronavirus and superbugs, undermine modern medicine,"" according to SWI's President, Erika Kurt. ""Leveraging the collective efforts of thousands of students and educators around the world gives us a real chance to tackle these challenges head on and provides an incredible STEM education in the process."" As COVID-19 has demonstrated, a lack of preparedness can take a huge human, financial, and educational toll, making the Small World Initiative's work critical to our fight against the next pandemic.

 

Differentiating itself from traditional science education programs, SWI's program provides original research opportunities rather than relying on cookbook experiments with predetermined results. Students are introduced to discovery-based research through the core research project of antibiotic discovery from soil microbes.

 

Through a series of experiments, students collect soil samples, isolate diverse bacteria, test their bacteria against clinically-relevant microorganisms, and characterize those showing inhibitory activity.

 

While most antibiotics come from soil and it remains a great place to look for new ones, finding new candidates requires screening thousands of samples.

 

SWI's approach provides the requisite platform to crowdsource antibiotic discovery by tapping into the power of thousands of student researchers concurrently addressing a global challenge.

 

This unique approach harnesses the power of active learning to achieve both educational and scientific goals. Since its inception, SWI has impacted hundreds of schools across the United States and around the world and helps them transform science education and promote antibiotic discovery through the curiosity and inventiveness of young scientists.

 

""SWI allows students to not just dream about using science to save lives, but to actually dig in and help save the world, starting at school,"" says Kurt. ""The program is an example of how groundbreaking research can start in the classroom."""

Volume 1

Acknowledgements

Small World Initiative Overview

Introduction: The Antibiotic Crisis

Section 1: Living on a Bacterial Planet
          Experiment 1: Devise a method to transfer microbes from a soil sample to a medium in the lab

Section 2: More than just “Dirt”
          Experiment 2: Find a local* soil environment you wish to sample

Section 3: Redefining “Growth” and “Culture”
          Experiment 3: Find a method to isolate single colonies of bacteria from your soil sample

Section 4: Bacteria Are What They Eat, Too
          Experiment 4: Choose your own media na dculture conditions

Section 5: Solid Versus Liquid Cultures
          Experiment 5: Isolate unique colonies to test for antibiotic production

Section 6: Meet the ESKAPE Pathogens
          Experiment 6: Understand the significance of the ESKAPE pathogens and using safe relatives in the lab

Section 7: Antibiotic Discovery, Structure, & Targets
          Experiment 7: Design a method to screen for antibiotic producers

Section 8: Getting to Know Your Isolates
          Experiment 8: Conduct initial identification of your antibiotic-producing isolate

Section 9: It All Comes Down to Chemistry
          Experiment 9: Test an organic extract of your isolate for antibiotic activity

Section 10: Resisting Antibiotics
          Experiment 10: Test your isolate’s resistance to common antibiotics

Section 11: “Classic” versus “Modern”
          Experiment 11: Conduct biochemical characterization of your isolates

Section 12: Bacteria in Context
          Experiment 12: Assess your isolate’s activity against eukaryotes, potential use as biological control, and ecological relationships with other organisms

Future Directions

Concluding Remarks

 

Volume 2

Acknowledgements

Lab Safety and Best Practices

Agarose Gel Electrophoresis

Aligning and Combining 16S rRNA Gene Sequences

Analyzing Organic Extracts for Antibiotic Production

Analyzing Sequences with BLAST Search 

Antibiotic Resistance Test

Catalase Test

Colony Morphology

Colony PCR

Fermentation

Gram Stain

MacConkey Agar Test

Making Glycerol Stocks

Methanol Extraction 

Obtaining Soil Sample 

Picking and Patching Colonies

Plating Soil Sample

Screen for Isolate Antibiotic Production #1 – Patch/Patch

Screen for Isolate Antibiotic Production #2 – Spread/Patch

Screen for Isolate Antibiotic Production #3 – Top Agar

Serial Dilutions

Silica Column Chromatography Protocol

Spread Plate 

Streak Plate

Sulfide and Indole Production and Motility

Thin Layer Chromatography (TLC) 

Typical Media Menu